Molecular Core
The Molecular Core supports research and education at EVMS by housing technologically advanced equipment for analysis of DNA, RNA and protein. To aid in using the equipment within the Molecular Core, classes and training sessions are scheduled at various times during the year. Consultation with the Core Director is available by appointment. Equipment available within the core includes:
Nucleic Acid Analysis:
- Nanodrop Spectrophotometers (1000 and 8000 models)
- Agilent 2100 Electrophoresis Bioanalyzer
- iSeq 100 Sequencing System
- Bio-Rad Real Time PCR Systems (Connect and CFX-96 models)
- Qubit 4 Fluorometer
Protein Analysis:
- LI-COR Odyssey XF (near-infrared (NIR) and enhanced chemiluminescence)
- LI-COR Odyssey CLx System (NIR)
- Spectra iD3 Max Multi-mode microplate reader (Colorimetric functionality only)
- BioTek Synergy HTX Multi-mode microplate reader
- BioTek EL808 plate reader
- Nanodrop Spectrophotometers (1000 and 8000 models)
- Qubit 4 Fluorometer
Gel Documentation Systems:
- Axygen
- Bio-Rad Gel Doc EZ
Other
- Refrigerated centrifuges (micro and preparatory)
- Heat blocks
- Speedvac
- Nucleofector
- Ultrasonicator (water-bath and Covaris ultrasonicator)
Fee-for-Service
- DNA sequencing (Sanger), until March 2023
- Custom experimental service
Contact: [email protected]; 757-446-5916
George L. Wright Jr. Center for Biomedical Proteomics
The George L. Wright Jr. Center for Biomedical Proteomics is dedicated to excellence in biomedical proteomics. The center provides proteomics support to investigators in the cancer research center and the general scientific community. Located within the Leroy T. Canoles Jr. Cancer Research Center, the Center for Biomedical Proteomics houses a variety of mass spectrometers and other instruments for protein isolation, separation, identification, characterization and data analysis.
- Thermo Q-Exactive Mass Spectrometer
- Thermo Orbitrap Fusion Lumos Mass Spectrometer
- HPLC, FPLC & UHPLC systems
- Fully Integrated Speed-Vac System for HPLC and Mass Spectrometry sample prep
- 2D-Electrophoresis System & Agilent 3100 OFFGEL Fractionator
- Thermo Fisher Scientific NanoDrop One Spectrophotometer
- Qualitative/Quantitative Proteome Analysis & De Novo sequencing and PTM characterization
- ArcturusXT Laser Capture Microdissection System
Location: Lester Hall, 4th floor; Contact: 757-446-5676, [email protected]
Biorepository and Histology Core
The College of American Pathologists (CAP) accredited biorepository provides for the procurement, identification, collection, storage and distribution of biospecimens for research purposes. The biorepository has dedicated personnel for specimen procurement and distribution, data entry, hardware and software management, reporting and regulatory tasks and histology services. The environmentally-controlled facility, which includes spaces for storage of specimens as well as lab work is part of the EVMS Pathology and Anatomy department.
Immunohistochemistry, tissue micro array construction and microscope slide scanning services are available along with routine and specialized tissue processing, sectioning and staining in the histology core of the EVMS Biorepository. Services and equipment within the Biorepository include:
- Subject consenting and specimen processing and procurement
- Tissue microarray design & construction
- Tissue processing, sectioning (microtomy & cryotomy) and high-resolution imaging using a Leica Aperio AT2 Digital Slide Scanner
- Routine and specialized staining including immunohistochemistry
Contact: 757-446-7910, [email protected]; [email protected]
Flow Cytometry Core
The EVMS Flow Cytometry Core Facility (Flow Core) provides access to flow cytometry instrumentation and expertise to support research at EVMS and the research community of Hampton Roads. Flow Cytometry is a technique for measurement of individual cells in a stream of fluid for cell phenotyping, cell cycle analysis, cell signaling, protein modification, etc.
Training can be scheduled with the Flow Cytometry Core Facility staff as needed. The staff is available to provide technical support in designing experiments, preparing samples and setting up the instrument.
Additionally, the Flow Core proudly introduces two state-of-the-art additions: the NanoString nCounter for high-throughput gene expression analysis and the ImageStream MK II, providing exceptional resolution for imaging flow cytometry.
- Cytek Aurora Flow Cytometer Analyzer
- BD FACSAria Fusion
• Amnis ImageStreamX Mark II Imaging Flow Cytometer - Meso Scale Discovery Meso Quick Plex multiplex reader
• Nexcelom Cellometer Auto 1000 cell counter - NanoString nCounter Multiplex Gene Expression System that allows for multiplex analysis of up to 800 RNA, DNA or target proteins in a sample
- VetScan HM5 Hematology Analyzer
- VetScan VS2 Chemical Analyzer
Location: Lewis Hall 3052; Contact: [email protected], [email protected]
Research and Infrastructure Service Enterprise (RISE)
RISE develops collaborative frameworks for conducting translational research in areas such as healthcare analytics and delivery, population health, clinical trials and community health disparities.
Microscopy and Imaging Core
The EVMS Microscopy and lmaging Facility and is available for a fee to institutions outside EVMS. The EVMS Microscopy and lmaging Core provides technical assistance in microscopy to all faculty, staff and students of the Medical School and access to upright and inverted microscopes, all equipped with cameras, as well as a JEOL 1200EXII TEM* and a Zeiss 880 LSM confocal*. Computers in the core are equipped with MetaMorph image analysis software.
- Fluorescence, Brightfield, DIC, polarized light Olympus upright and inverted microscopes with cameras
- Zeiss in vivo and in vitro 880 Laser Scanning Confocal Microscope, with incubation chamber and Zen Black software
- Zeiss Axio Observer Inverted Fluorescence Microscope, Zen Blue software
- Keyence BZ-X All-in-One Fluorescence Microscope
- JEOL 1200EXll transmission electron microscope with an AMT XR111 digital camera for ultrastructural examination of biological tissue.
- Computers equipped with image analysis software (MetaMorph)
- ONI NanoImager Microscope
- Nikon Coolscope slide reader
Location: Lewis Hall 3084; Contact: 757-446-5658, [email protected]
Researchers@EVMS
Faculty can use this tool to locate research collaborators and students can use it to locate research mentors. View some instructional videos.
Pre-clinical resources (more information here)
Vivarium
The AAALAC accredited animal research facility located inside of the EVMS campus covers a space of approximately 16,000 ft2. The facility is able to house multiple species. BSL-2 rooms are available for use, if needed, and the animals are cared for by a full-time veterinarian with trained support staff. The vivarium is set up to assist the investigators with breeding programs, diet studies, drug delivery, and surgery.
The facility contains a modern surgical suite with 2 operating rooms, a scrub room, a prep room, and is capable of housing and working with most species. Imaging via 3D and 4D ultrasound, X-ray, and an IVIS Lumina imaging system is available. Additionally, the X-RAD320 a high- end, fully shielded, biological irradiation cabinet for use in Basic Sciences research is newly available. There are separate rooms available for procedures in each area of the vivarium. A necropsy room with down draft table is available for use. There are 2 separate cage wash units, as well as multiple autoclaves for use.
Management of animal facilities
The Division of Comparative Medicine (CompMed) supports every aspect of the animal care and use program in its role as the institutional entity responsible for animal procurement, animal husbandry, veterinary care and health surveillance, preventative medicine programs, animal quarantine, oversight and maintenance of the animal facilities and related equipment, personnel training, and specialized technical support.
CompMed, along with the Institutional Animal Care and Use Committee (IACUC), is charged with guiding the animal care and use program in
support of research and compliance with all federal, state, local, and EVMS guidelines, policies and regulations. CompMed is managed by SoBran Bioscience, Inc., a nationally recognized provider of support services for biomedical research.
The CompMed staff consists of the Attending Veterinarian (AV)/Clinical Veterinarian (CV), Project Manager (PM) and supporting technical and research specialist staff. The AV reports to the EVMS Institutional Official (IO) on all matters related to animal care and use and participates in the IACUC. The IACUC members are appointed by the Dean/Provost, whom the IO reports to.
Core laboratory resource sharing agreement between Virginia research institutions
EVMS is one of seven academic research institutions in Virginia that have signed a memorandum of understanding to share core facilities and resources in an effort to advance the research enterprise in the Commonwealth.
MedDRA
EVMS has an institutional license to MedDRA (Medical Dictionary for Regulatory Activities), a clinically-validated international medical terminology used by regulatory authorities, including the FDA. It is also used by industry, academics, health professionals and other organizations to communicate medical information.
SciFinder
Provides access to research in chemistry and related sciences from Chemical Abstracts Service, including the world's largest collection of substance information, the CAS REGISTRY. ODU students, faculty and staff must REGISTER for their own user account in order to login to the database. Helpful SciFinder-n FAQs provided here.