Figure 3: Repetitive motifs are extensively shared across spidroins.
(a) Bar graph comparing the number of shared (gold) versus private (dark gray) distinct repetitive motifs observed in each spidroin. (b–g) Circos plots illustrate sharing of motif sequences among N. clavipes spidroins, specifically showing sharing of sequences belonging to known motif groups (b), known motif groups with novel N. clavipes variants (c), novel motif groups (d), and unassigned motifs (e). In these plots, genes are arrayed around the circle, and links are drawn to connect similar motif sequences that occur in both genes. (f) Circos plot showing the extensive sharing of motifs between novel FLAG-b (VeSp) and the other N. clavipes spidroins, supporting classification of FLAG-b as a spidroin. (g) The Circos plot of FLAG-a shows a similar distribution of motif sharing as seen for FLAG-b and further highlights the lack of motif sharing of either gene with AgSp-d.