Challenges in somatic variant calling include a lack of long-read variant callers and of publicly available benchmarking datasets. We developed DeepSomatic, a somatic variant caller for short- and long-read technologies, and created seven somatic variant benchmarks derived from cancer cell lines, which we make available as a public database: CASTLE-panel.

Precise control of transgene expression remains a challenge in engineering primary cells for diverse applications. We developed DIAL, a promoter editing framework that transmits transient inputs into stable setpoints of expression in primary cells and human induced pluripotent stem cells, paving the way for predictable programming of gene circuits in therapeutically relevant cells.

Transient expression of a transcription activator-like effector (TALE)-based epigenetic editor demonstrates durable therapeutic silencing in non-human primates.

Spermatid-like haploid stem cells produce genome-edited farm animals in one generation.