The complex spatiotemporal signaling of human neural tube development is successfully engineered in a device using microfluidic gradients, creating a high-fidelity model of the developing human central nervous system.

Filter-aided expansion proteomics facilitates spatial proteomics at subcellular resolution by integrating tissue expansion, imaging-guided microdissection and filter-aided in-gel digestion. This combination enhances the resolution, throughput and reproducibility for data-independent acquisition-based mass spectrometry analysis.

CRISPR screens are widely used to identify essential genes and potential drug targets, but typical setups were designed for the study of highly proliferative cancer cell lines. Now, a protocol, using inducible Cas9, enables screens of non-proliferative cell states including senescence, quiescence and terminal differentiation, thereby expanding the applicability of CRISPR screens.

Here, the authors provide a platform for inducible CRISPR–Cas9 screening to interrogate non-proliferative cellular states.

ANPELA is a software package to compare and assess the performance of different workflows for processing single-cell proteomic data, ensuring the user selects the most appropriate processing workflow for their experimental design question.

The preparation of multicomponent viscoelastic self-enhancing sono-inks, synthesized as phase-transition reversible acoustic absorbers, enables acoustic volumetric printing beneath diverse tissue types in optically scattering media.

In mass photometry, the optical contrast generated by individual molecules at a glass–water interface enables mass-resolved quantification of biomolecular mixtures. This protocol describes how to optimize and validate this method.

Protocol for fabricating synthetic viscoelastic antigen-presenting cells and their application in T cell engineering. These synthetic cells support robust T cell activation and expansion and improve chimeric antigen receptor transduction efficiency.

Filter-aided expansion proteomics facilitates spatial proteomics at subcellular resolution by integrating tissue expansion, imaging-guided microdissection and filter-aided in-gel digestion. This combination enhances the resolution, throughput and reproducibility for data-independent acquisition-based mass spectrometry analysis.

The complex spatiotemporal signaling of human neural tube development is successfully engineered in a device using microfluidic gradients, creating a high-fidelity model of the developing human central nervous system.

CRISPR screens are widely used to identify essential genes and potential drug targets, but typical setups were designed for the study of highly proliferative cancer cell lines. Now, a protocol, using inducible Cas9, enables screens of non-proliferative cell states including senescence, quiescence and terminal differentiation, thereby expanding the applicability of CRISPR screens.

Here, the authors provide a platform for inducible CRISPR–Cas9 screening to interrogate non-proliferative cellular states.

Protocol for generating spatially patterned, human neural tube- and forebrain-like structures, by using a microfluidic device to impose orthogonal and independent chemical gradients on human pluripotent stem cells.