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Screening, identification, and characterization of a novel saccharide-stimulated β-glycosidase from a soil metagenomic library

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Abstract

MeBglD2, a β-glycosidase that is highly activated in the presence of various monosaccharides and disaccharides, was isolated from a soil metagenomic library. MeBglD2 had not only β-glucosidase activity but also β-galactosidase and β-fucosidase activities. MeBglD2 β-glucosidase activity increased in a cellobiose concentration-dependent manner and was not inhibited by a high concentration of d-glucose or cellobiose. MeBglD2 β-glucosidase and β-fucosidase activities were activated by various monosaccharides and disaccharides including d-glucose, d-xylose, d-galactose, maltose, and cellobiose. The saccharification yield of rice straw using Trichoderma reesei cellulase was improved by the addition of MeBglD2. These results show that MeBglD2 can be used to improve plant biomass saccharification, because both substrates and products can activate its enzymatic activity.

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Acknowledgments

We thank Dr. Nobutada Kimura and Dr. Hikaru Suenaga (AIST) for the metagenomic library and technical support. Alkaline-treated rice straw was kindly provided by Dr. Yoshinori Kobayashi (Japan Bioindustry Association).

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  1. Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan

    Tomohiko Matsuzawa & Katsuro Yaoi

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  1. Tomohiko Matsuzawa
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  2. Katsuro Yaoi
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Correspondence to Tomohiko Matsuzawa or Katsuro Yaoi.

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This study was funded by JSPS KAKENHI (Grant-in-Aid for Young Scientists B) Grant Number 26850067.

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Matsuzawa, T., Yaoi, K. Screening, identification, and characterization of a novel saccharide-stimulated β-glycosidase from a soil metagenomic library. Appl Microbiol Biotechnol 101, 633–646 (2017). https://doi.org/10.1007/s00253-016-7803-2

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