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Larvicidal efficacy of Adhatoda vasica (L.) Nees against the bancroftian filariasis vector Culex quinquefasciatus Say and dengue vector Aedes aegypti L. in in vitro condition

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Abstract

The larvicidal activities of methanolic fractions from Adhatoda vasica leaf extracts were investigated against the bancroftian filariasis vector Culex quinquefasciatus and dengue vector Aedes aegypti. The results indicated that the mortality rates was high at 100, 150, 200 and 250 ppm of methanol extract of fractions III with R f value 0.67 and methanol extract of fraction V with R f value 0.64 of A. vasica against all the larval instars of C. quinquefasciatus and A. aegypti. The result of log probit analysis (at 95% confidence level) revealed that lethal concentration, LC50 and LC90 values were 106.13 and 180.6 ppm for fraction III, 110.6 and 170 ppm for fraction V of C. quinquefasciatus. And, the LC50 and LC90 values were 157.5 and 215.5 ppm for fraction III of A. aegypti and 120 and 243.5 ppm for the fraction V of A. aegypti, respectively. All the tested fractions proved to have strong larvicidal activity (doses from 100 to 250 ppm) against C. quinquefasciatus and A. aegypti. In general, second instar was more susceptible than the later instar. The results achieved suggest that, in addition to their ethnopharmacology value, A. vasica may also serve as a natural larvicidal agent.

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Acknowledgments

This research was supported by the MS University seed money grant (Grant No. MSU/R/cir/seedmoney/2010-2011). The authors express gratitude to Dr. S. Kim, Chonbuk National University, Republic of Korea for providing valuable reprints and related articles. We thank Mr. K. Karthik, Technical Assistant, for his technical assistance and insect rearing.

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  1. Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Centre for Excellence in Environmental Sciences (SPKCES), Manonmaniam Sundaranar University, Alwarkurichi-627 412, Tirunelveli, Tamil Nadu, India

    Annamalai Thanigaivel, Rajamanickam Chandrasekaran, Kannan Revathi, Selvamathiazhagan Nisha, Subbiah Sathish-Narayanan, Suyambulingam Arunachalam Kirubakaran & Sengottayan Senthil-Nathan

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  1. Annamalai Thanigaivel
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Thanigaivel, A., Chandrasekaran, R., Revathi, K. et al. Larvicidal efficacy of Adhatoda vasica (L.) Nees against the bancroftian filariasis vector Culex quinquefasciatus Say and dengue vector Aedes aegypti L. in in vitro condition. Parasitol Res 110, 1993–1999 (2012). https://doi.org/10.1007/s00436-011-2728-2

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