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Rationale for developing novel mosquito larvicides based on isofuranodiene microemulsions

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Abstract

Isofuranodiene is a bioactive furanosesquiterpene occurring as the major volatile compound in the essential oil from Smyrnium olusatrum (Apiaceae). It has a notable potentiality to be used as a botanical insecticide. However, its low solubility together with susceptibility to oxidation and thermal degradation limits its application on a wide scale. To face these challenges, isofuranodiene was encapsulated in stable microemulsions (MEs) at two concentrations (ME 750: 0.75%, ME 375: 0.375%). We assessed the larvicidal activity in the short term and after several days of exposure of pure isofuranodiene and its MEs against the filariasis vector Culex quinquefasciatus. The impact of ME-based larvicide at sublethal concentrations on adult emergence was evaluated. Finally, to shed light on potential ecotoxicology risks for nontarget invertebrates, MEs were tested on the aquatic microcrustacean Daphnia magna and on the earthworm Eisenia fetida. Pure isofuranodiene and S. olusatrum leaf essential oil, used as controls, showed their potential as mosquito larvicidal agents (LC50 of 29.2 and 18.6 μL L−1, respectively). Isofuranodiene formulated in ME 750 was effective against C. quinquefasciatus larvae, leading to significant larval mortality over time and a marked decrease in adult emergence. Both isofuranodiene MEs showed little impact on the nontarget aquatic microcrustacean Daphnia magna (ME 750 and ME 375: mortality < 19% after 48 h of exposure at 32 mL L−1 and 20 mL L−1, respectively) as well as on earthworms (ME 750 and ME 375: no mortality and mortality < 7% after 14 days of exposure to 1000 mg kg−1, respectively), outlining their potential employ in vector control operations.

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Acknowledgements

Roman Pavela would like to thank the Ministry of Agriculture of the Czech Republic for its financial support concerning botanical pesticides and basic substances research (Project MZE-RO0418).

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  1. Roman Pavela and Lucia Pavoni have contributed equally to this work.

Authors and Affiliations

  1. Crop Research Institute, Drnovska 507, Ruzyne, 161 06, Prague 6, Czech Republic

    Roman Pavela

  2. Department of Plant Protection, Czech University of Life Sciences Prague, Kamycka 129, Suchdol, 165 00, Prague 6, Czech Republic

    Roman Pavela

  3. School of Pharmacy, University of Camerino, 62032, Camerino, Italy

    Lucia Pavoni, Giulia Bonacucina, Marco Cespi, Loredana Cappellacci, Riccardo Petrelli & Filippo Maggi

  4. Laboratory of Agricultural Zoology and Entomology, Department of Crop Science, Agricultural University of Athens, 75 Iera Odos str., Athens, Greece

    Nickolas G. Kavallieratos

  5. Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124, Pisa, Italy

    Giovanni Benelli

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Pavela, R., Pavoni, L., Bonacucina, G. et al. Rationale for developing novel mosquito larvicides based on isofuranodiene microemulsions. J Pest Sci 92, 909–921 (2019). https://doi.org/10.1007/s10340-018-01076-3

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