Abstract
High temperature has a significant impact on the growth and development of plants, especially pioneer herbaceous plants such as Paspalum wettsteinii, which are adapted to harsh environments. We investigated the root morphology and transcriptome of P. wettsteinii under 35 °C, 40 °C, and 45 °C heat stress treatments to explore its response mechanism under high-temperature stress. Under high-temperature stress, P. wettsteinii adapted to heat by appropriately increasing the root surface area and total branching number while reducing the root length to maintain basic growth and improve stress tolerance. Transcriptomic analysis revealed significant changes in genes related to heat shock proteins (HSPs), flavonoid synthesis, and fatty acid metabolism to different degrees. With the increase in the temperature of heat stress, a large number of HSP genes in P. wettsteinii were upregulated to adapt to high temperature, while a large number of genes involved in flavonoid synthesis and fatty acid metabolism were downregulated, providing insight into the response to high temperature. These findings revealed the changes in root morphology and key genes in P. wettsteinii under high-temperature stress and provide important references for studying the physiological and molecular regulation of plant high-temperature stress.
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Data availability
The datasets generated during and analysed during the current study are available in the NCBI (SRA accession: PRJNA801753) repository.
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Acknowledgements
We thank the Guizhou Provincial Department of Education Natural Science Research Project (QJJ[2022]076), Guizhou Provincial Science and Technology Projects (QKHPTRC[2021]5636), and the National Natural Science Foundation of China (32060391, 31560664) for funding our research.
Funding
This work was supported by Guizhou Provincial Department of Education Natural Science Research Project (QJJ[2022]076), Guizhou Provincial Science and Technology Projects (QKHPTRC[2021]5636), and the National Natural Science Foundation of China (32060391, 31560664).
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ZJY: Writing—original draft and interpreted the data HLJ: Conduct of experiments WPC: Planning and monitoring experiments ZLL: Planning and monitoring experiments. All authors read and approved the final manuscript.
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Zhou, J., Huang, L., Wang, P. et al. Root morphology and transcriptomic analyses of the response to heat stress in Paspalum wettsteinii hack. Genet Resour Crop Evol 71, 3219–3239 (2024). https://doi.org/10.1007/s10722-023-01828-w
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DOI: https://doi.org/10.1007/s10722-023-01828-w