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Identification and characterization of uncoupling protein in heart and muscle mitochondria of canary birds

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Abstract

An uncoupling protein (cUCP) was identified in heart and skeletal muscle mitochondria of canary birds. cUCP was immunodetected using polyclonal antibodies raised against murine UCP2. Its molecular mass was similar to those of mammalian UCPs (32 kDa). The activity of cUCP was stimulated by palmitic acid (PA) and inhibited by GTP mainly in state 3 respiration. Additions of PA augmented state 4 respiration and lowered the ADP/O ratio. Thus, the activity of cUCP diverted energy from oxidative phosphorylation in state 3 respiration. cUCP in heart and skeletal muscles of canary birds might have implications in thermogenesis as well as protection against free radical production.

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Authors and Affiliations

  1. Department of Animal Physiology and Development, Adam Mickiewicz University, Poznań, ul., Umultowska 89, Poland

    Malgorzata B. Slocinska

  2. Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Kent Ridge, Singapore

    Zakaria Ali Moh. Almsherqi & Yuru Deng

  3. Laboratory of Bioenergetics, Center of Oxygen Research and Development, Department of Life Sciences, Institute of Chemistry B6c, University of Liege, Sart Tilman, Liege, Belgium

    Francis E. Sluse & Rachel Navet

Authors
  1. Malgorzata B. Slocinska
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  2. Zakaria Ali Moh. Almsherqi
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  3. Francis E. Sluse
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  4. Rachel Navet
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  5. Yuru Deng
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Corresponding authors

Correspondence to Malgorzata B. Slocinska or Yuru Deng.

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Malgorzata B. Slocinska and Zakaria Ali Moh Almsherqi contributed equally to this work

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Slocinska, M.B., Almsherqi, Z.A.M., Sluse, F.E. et al. Identification and characterization of uncoupling protein in heart and muscle mitochondria of canary birds. J Bioenerg Biomembr 42, 345–353 (2010). https://doi.org/10.1007/s10863-010-9303-x

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  • DOI: https://doi.org/10.1007/s10863-010-9303-x

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