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Cloning and expression analysis of the gene encoding fibrinogen-like protein A, a novel regeneration-related protein from Apostichopus japonicus

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Abstract

Fibrinogen-like protein A (FGLA), a member of the fibrinogen-related protein superfamily, exists in different tissues of vertebrates and invertebrates. FGLA plays crucial roles including innate immune response, blood clotting and regeneration. In this study, the fibrinogen-like protein A (fglA) was cloned from Apostichopus japonicus using rapid amplification of cDNA ends PCR techniques. The cDNA sequence of fglA is 1,524 bp with a 849 bp open reading frame encoding a polypeptide of 282 amino acids, with an N-terminal signal peptide and a conserved C-terminal domain. Bioinformatic analysis revealed that the predicted molecular weight of the whole protein is 31.9 kDa and it has an isoelectric point of 5.64. In-situ hybridization demonstrated that fglA is widely distributed in body wall, intestines, longitudinal muscles and respiratory tree. The expression levels of fglA during different regeneration stages in the body wall, intestine and respiratory trees were analyzed by real-time PCR. The expression of fglA gradually increased within 1 h in body wall, and reached a plateau before decreasing to the basal level. This indicates that fglA is associated with the regeneration of Apostichopus japonicus.

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Acknowledgments

We thank Professor Liu Weidong of the Liaoning Ocean and Fisheries Science Research Institute for assistance with the experiment.

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Authors and Affiliations

  1. College of Life Sciences, Liaoning Normal University, Dalian, 116081, People’s Republic of China

    Yang Wu, Feng Yao, Yanli Mei, Bing Chu, Cheng Cheng, Yan Liu, Xuejie Li & Lin Hou

  2. Department of Biology, Dalian Medical University, Dalian, 116044, People’s Republic of China

    Xiangyang Zou

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  1. Yang Wu
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  2. Feng Yao
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  3. Yanli Mei
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  8. Xiangyang Zou
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  9. Lin Hou
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Correspondence to Lin Hou.

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Wu, Y., Yao, F., Mei, Y. et al. Cloning and expression analysis of the gene encoding fibrinogen-like protein A, a novel regeneration-related protein from Apostichopus japonicus . Mol Biol Rep 41, 2617–2627 (2014). https://doi.org/10.1007/s11033-014-3120-y

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  • DOI: https://doi.org/10.1007/s11033-014-3120-y

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