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Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus)

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Abstract

Channel catfish (Ictalurus punctatus) is the primary culture species in the US along with its hybrid made with male blue catfish, I. furcatus. In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin promoter (βactin) was inserted into channel catfish. The objectives of this study were to determine the effectiveness of βactin-D5D for improving n-3 fatty acid production in F1 transgenic channel catfish, as well as examine pleiotropic effects on growth, proximate analysis, disease resistance, and other performance traits. Transgenic F1 channel catfish showed a 33% increase in the relative proportion of n-3 fatty acids coupled with a 15% decrease in n-6 fatty acids and a 17% decrease in n-9 fatty acids when compared to non-transgenic full-siblings (P < 0.01, P < 0.01, P < 0.01). However, while the relative proportion of n-3 fatty acids was achieved, the total amount of fatty acids in the transgenic fish decreased resulting in a reduction of all fatty acids. Insertion of the βactin-D5D transgene into channel catfish also had large effects on the body composition, and growth of channel catfish. Transgenic channel catfish grew faster, were more disease resistant, had higher protein and moisture percentage, but lower fat percentage than full-sib controls. There were sex effects as performance changes were more dramatic and significant in males. The βactin-D5D transgenic channel catfish were also more uniform in their fatty acid composition, growth and other traits.

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Acknowledgements

This project was supported by the Ocean University of China-Auburn University Joint Center. The authors thank Dr. Donald Allen Davis of the School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, Alabama, 36849, USA for providing lab space for proximate analysis and sample preparation.

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Authors and Affiliations

  1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China

    Yingqi Huang & Yiliu Zhang

  2. Department of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

    William Bugg

  3. Department of Biological Sciences, Florida State University, Tallahassee, FL, 32304, USA

    Max Bangs

  4. Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, 02115, USA

    Guyu Qin

  5. Muckleshoot Indian Tribe Department of Fisheries, 39015-A 172nd Ave SE, Auburn, WA, 98092, USA

    David Drescher

  6. Department of Biological Sciences, University At Buffalo, Buffalo, NY, 14260, USA

    Nathan Backenstose

  7. School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 203 Swingle Hall, Auburn, AL, 36849, USA

    Chia Chen Weng, Cuiyu Lu, Xiaoli Ma, Zachary Taylor, Yujia Yang, Tao Zhou, Jingping Guo & Rex A. Dunham

  8. Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt

    Karim Khalil

  9. Department of Civil and Environmental Engineering, Auburn University, Auburn, AL, 36849, USA

    Sheng Dong

  10. Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt

    Ahmed Elaswad

  11. Department of Biochemistry, University of Washington, Seattle, WA, 98195-7350, USA

    Zhi Ye

  12. Department of Aquacultures and Technology, Can Tho Technical Economic College, Can Tho City, Vietnam

    Khoi Vo

  13. College of Fisheries and Ocean Sciences, University of the Philippines Visayas, Miagao, 5023, Iloilo, Philippines

    Rhoda Mae Simora

  14. KnipBio, 110 Canal St, Lowell, MA, 01852, USA

    Guillaume Salze

  15. MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Zhenkui Qin

  16. Biosystems Engineering Department, Auburn University, Auburn, AL, 36849, USA

    Yi Wang

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Huang, Y., Bugg, W., Bangs, M. et al. Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus). Transgenic Res 30, 185–200 (2021). https://doi.org/10.1007/s11248-021-00242-1

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