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Study on the mechanism of let-7a-5p in regulating the proliferation in cervical cancer cells

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A Correction to this article was published on 07 July 2022

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Abstract

Purpose

To explore the regulatory effect of let-7a-5p/TGFBR1/Smad3 on the proliferation activity of cervical cancer cells.

Methods

The difference in let-7a-5p expression between normal people and patients with cervical cancer was detected by miREIA assay. The differences of let-7a-5p expression between cervical cancer cell line C33a and adjacent normal epithelial cell line HUCEC were determined by qRT-PCR.

Results

miREIA result showed that let-7a-5p concentrations were 178.5 ± 24.3 μg/L in healthy individuals and 106.1 ± 14.8 μg/L in cervical cancer patients (P = 0.0002). qRT-PCR showed that let-7a-5p in cervical cancer tissue (0.57 ± 0.03) was lower than that in adjacent normal tissue (0.84 ± 0.04, P = 0.0107). Compared with normal cervical epithelial cells (HUCEC), the expression of let-7a-5p was lower in cervical cancer cells (C33a, Hela, P = 0.0001). The results of CCK-8 and EDU detection showed that activation of let-7a-5p inhibited the proliferation of C33a (P = 0.00130, P << 0.0001) and Hela (P = 0.00254, P = 0.0066) cells. According to the analysis using Starbase V2.0 online database, let-7a-5p could target TGFβR1 in cervical cancer cell lines, and the let-7a-5p mimic reduces the mRNA expression level of TGFβR1 in cervical cancer cell C33a (P = 0.0067). Western blot results showed that TGFBR1 expression significantly decreased in cervical cancer cells after let-7a-5p mimic treatment (P = 0.0048) and significantly increased after let-7a-5p mimic inhibitor treatment (P = 0.0003).

Conclusions

let-7a-5p represents the independent novel anti-oncogenes in cervical cancer, which can regulate TGF-β1/TGFBR1/pSmad3 cell pathway and interfere with the proliferation of cervical cancer cells. Therefore, let-7a-5p can serve as a novel potential therapeutic target for the treatment of cervical cancer.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This study was funded by General Project of Fujian Natural Science Foundation (2017J01254) and Joint Fund Project of Provincial Science and Technology Innovation, 2020 (No. 2020Y9153).

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Author notes

  1. Zhiqin Chen and Jiahui Qiu contributed equally and should be listed as co-first authors.

Authors and Affiliations

  1. Departments of Gynecology, Shengli Clinical Medical College of Fujian Medical University and Fujian Provincial Hospital, No. 134 East Street, Gulou District, Fuzhou, 350001, Fujian Province, China

    Zhiqin Chen, Yuling Gao, Qin Lu & Hong Shi

  2. Departments of Traditional Chinese Medicine, Fujian Obstetrics and Gynecology Hospital/Fujian Maternal and Child Health Care Hospital/College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, 350000, China

    Jiahui Qiu

  3. Department of Pathology, Shengli Clinical Medical College of Fujian Medical University & Fujian Provincial Hospital, Fuzhou, 350001, China

    Ying Lin

Authors
  1. Zhiqin Chen
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  2. Jiahui Qiu
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  3. Yuling Gao
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  4. Qin Lu
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  5. Ying Lin
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  6. Hong Shi
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Contributions

ZC and HS conceived and designed the study, and drafted the manuscript. ZC, JQ, YG, QL and YL collected, analyzed and interpreted the experimental data. JQ and HS revised the manuscript for important intellectual content. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Hong Shi.

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Conflict of interest

The authors declare that they have no financial conflicts of interest.

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Written informed consent was obtained from patients with approval by the Shengli Clinical Medical College of Fujian Medical University and Fujian Provincial Hospital.

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Written informed consent was obtained from patients with approval by the Shengli Clinical Medical College of Fujian Medical University and Fujian Provincial Hospital.

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Chen, Z., Qiu, J., Gao, Y. et al. Study on the mechanism of let-7a-5p in regulating the proliferation in cervical cancer cells. Clin Transl Oncol 24, 1631–1642 (2022). https://doi.org/10.1007/s12094-022-02810-1

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  • DOI: https://doi.org/10.1007/s12094-022-02810-1

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