Thanks to visit codestin.com
Credit goes to link.springer.com

Skip to main content
Springer Nature Link
Log in

N Fertilizer Dose-Dependent Efficiency of Serratia spp. for Improving Growth and Yield of Upland Rice (Oryza sativa L.)

  • Research
  • Published:
International Journal of Plant Production Aims and scope Submit manuscript

Abstract

Serratia spp., strain BRM 32114, is a plant growth-promoting rhizobacteria that show strong potential to solve two major challenges of upland rice production in a no-tillage system: initial vigor and grain yield improvement. No-till practices dictate the sustainability of cropping systems as they improve precipitation use, sequester C, mitigate atmospheric CO2 enrichment, restore soil health, and stimulate interest in crop diversity and rotation design. This study was intended to analyze the effect of the Serratia spp. on growth promotion and grain yield improvement in upland rice, under four N rates. Two field experiments were conducted during growing seasons 2015/2016 and 2016/2017, in two different experimental areas managed under a no-tillage system in the Brazilian Cerrado soil. The experimental design was a complete randomized block in a 4 × 2 factorial scheme, with four replications. Treatments comprised of four N fertilizer rates (0, 40, 80 and 120 kg N ha−1) with or without BRM 32114. In BRM 32114 treatment, rice seeds were microbiolized and bacterial suspension were sprayed at soil/plant in the field on the 7th and 15th DAS (day after sowing). Morphophysiological (gas exchange, shoot nutrients content and biomass production), and agronomic (grain yield and its components) traits were estimated. Results revealed increase in stomatal conductance (~ 20%); N, Ca and Mg (7, 11 and 9%) contents; shoot dry matter (8%); number of grain per plant (17%); mass of 1000 grains (2%) and yield (~ 22%) in BRM 32114-treated rice and cultivated in soil fertilized with 0, 40 and 80 kg N ha−1. However, the efficacy of this strain was reduced when combined with the highest rate of N (120 kg ha−1) added to the soil. It is possible to get higher yields levels of upland rice, cultivated under no tillage system, with the complimentary use of Serratia sp., strain BRM 32114, than the application of chemical fertilizers alone. The improvement in soil health could be extra benefit for fertilizer’ savings.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+
from £29.99 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price includes VAT (United Kingdom)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

Explore related subjects

Discover the latest articles, books and news in related subjects, suggested using machine learning.

References

  • Ahemad, M., & Kibret, M. (2014). Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University, 26, 1–20.

    Article  Google Scholar 

  • Araújo, A. E. S., Baldani, V. L. D., Galisa, P. S., Pereira, J. A., & Baldani, J. I. (2013). Response of traditional upland rice varieties to inoculation with selected diazotrophic bacteria isolated from rice cropped at the Northeast region of Brazil. Applied Soil Ecology, 64, 49–55.

    Article  Google Scholar 

  • Baldani, V. L. D., Baldani, J. I., & Döbereiner, J. (2000). Inoculation of rice plants with the endophytic diazotrophs Herbaspirillum seropedicae and Burkholderia spp. Biology and Fertility of Soils, 30, 485–491.

    Article  Google Scholar 

  • Baris, O., Sahin, F., Turan, M., Orhan, F., & Gulluce, M. (2014). Use of plant-growth-promoting rhizobacteria (PGPR) seed inoculation as alternative fertilizer inputs in wheat and barley production. Communications in Soil Science and Plant Analysis, 45, 2457–2467.

    Article  CAS  Google Scholar 

  • Bernier, J., Atlin, G. N., Et, A. L., Serraj, R., Kumar, A., & Spaner, D. (2008). Breeding upland rice for drought resistance. Journal of the Science of Food and Agriculture, 88, 927–939.

    Article  CAS  Google Scholar 

  • Berry, J., Beerling, D., & Franks, P. (2010). Stomata: Key players in the earth system, past and present. Current Opinion in Plant Biology, 13, 232–239.

    Article  Google Scholar 

  • Buresh, R. J., Reddy, K. R., & van Kessel, C. (2008). Nitrogen transformations in submerged soils. In J. S. Schepers & W. R. Raun (Eds.), Nitrogen in agricultural systems. Agronomy monograph, Madison (Vol. 49, pp 401–436).

  • De Freitas, J. R., & Germida, J. J. (1990). Plant growth promoting rhizobacteria for winter wheat. Canadian Journal of Microbiology, 36, 265–272.

    Article  Google Scholar 

  • Donagema, G. K., Campos, D. V. B., Calderano, S. B., & Teixeira, W. G. (2011). Manual de métodos de análise de solo (segunda ed.). Rio de Janeiro: Embrapa Solos.

    Google Scholar 

  • Fageria, N. K. (2014). Nitrogen management in crop production. Boca Raton: CRC Press.

    Book  Google Scholar 

  • Fageria, N. K., Moreira, A., & Coelho, A. M. (2011). Yield and yield components of upland rice as influenced by nitrogen sources. Journal of Plant Nutrition, 34, 361–370.

    Article  CAS  Google Scholar 

  • Fernández-Bidondo, L., Silvani, V., Colombo, R., Pérgola, M., Bompadre, J., & Godeas, A. (2011). Pre-symbiotic and symbiotic interactions between Glomus intraradices and two Paenobacillus species isolated from AM propagules. In vitro and in vivo assays with soybean (AG043RG) as plant host. Soil Biology & Biochemistry, 43, 1866–1872.

    Article  CAS  Google Scholar 

  • Filippi, M. C. C., Silva, G. B., Silva-Lobo, V. L., Cortes, M. M. C. B., Moraes, A. J. G., & Prabhu, A. S. (2011). Leaf blast (Magnaporthe oryzae) suppression and growth promotion by rhizobacteria on aerobic rice in Brazil. Biological Control, 58, 160–166.

    Article  Google Scholar 

  • Garcia de Salamone, I. E., Dobereiner, J., Urquiaga, S., & Boddey, R. M. (1996). Biological nitrogen fixation in Azospirillum strain-maize genotype associations as evaluated by the 15N isotope dilution technique. Biology and Fertility of Soils, 23, 249–256.

    Article  CAS  Google Scholar 

  • Gyaneshwar, P. G., Mathan, N., Reddy, P. M., Reinhold-Hurek, B., & Ladha, J. K. (2001). Endophytic colonization of rice b a diazotrophic strain of Serratia marcescens. Journal of Bacteriology, 183, 2634–2645.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Horie, T., Matsuura, S., Takai, T., Kuwasaki, K., Ohsumi, A., & Shiraiwa, T. (2006). Genotypic difference in canopy diffusive conductance measured by a new remote-sensing method and its association with the difference in rice yield potential. Plant, Cell & Environment, 29, 653–660.

    Article  Google Scholar 

  • Isawa, T., Yasuda, M., Awasaki, H., Minamisawa, K., Shinozaki, S., & Nakashita, H. (2010). Azospirillum sp. strain B510 enhances rice growth and yield. Microbes and Environments, 25, 58–61.

    Article  PubMed  Google Scholar 

  • Kado, C. J., & Heskett, M. G. (1970). Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas and Xanthomonas. Phytopathology, 60, 969–976.

    Article  CAS  PubMed  Google Scholar 

  • Karlidag, H., Esitken, A., Turan, M., & Sahin, F. (2007). Effects of root inoculation of plant growth promoting rhizobacteria (PGPR) on yield, growth and nutrient element contents of leaves of apple. Scientia Horticulturae, 114, 16–20.

    Article  CAS  Google Scholar 

  • Kikuta, M., Yamamoto, Y., Pasolon, Y. B., Rembon, F. S., Miyazaki, A., & Makihara, D. (2016). How growth and yield of upland rice vary with topographic conditions: a case of slash-and-burn rice farming in South Konawe Regency, Southeast Sulawesi Province, Indonesia. Tropical Agriculture and Development, 60, 162–171.

    Google Scholar 

  • Kuan, K. B., Othman, R., Rahim, K. A., & Shamsuddin, Z. H. (2016). Plant growth-promoting rhizobacteria inoculation to enhance vegetative growth, nitrogen fixation and nitrogen remobilisation of maize under greenhouse conditions. PLoS One, 11, e0152478.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kumar, V., & Ladha, J. K. (2011). Direct seeding of rice: recent developments and future research needs. Advances in Agronomy, 111, 297–396.

    Article  Google Scholar 

  • Ladha, J. K., Pathak, H., Krupnik, T. J., Six, J., & Van Kessel, C. (2005). Efficiency of fertilizer nitrogen in cereal production: Retrospect and prospects. Advances in Agronomy, 87, 85–156.

    Article  CAS  Google Scholar 

  • Lopes, V. R. (2013). Melhoramento genético de cana-de-açúcar em associação com bactérias promotoras do crescimento vegetal. Dissertação, Universidade Federal do Paraná: Curitiba.

    Google Scholar 

  • Lucas, J. A., Ramos-Solano, B., Montes, F., Ojeda, J., Megías, M., & Gutiérrez‐Mañero, F. J. (2009). Use of two PGPR strains in the integrated management of blast disease in rice (Oryza sativa) in Southern Spain. Field Crops Research, 114, 404–410.

    Article  Google Scholar 

  • Mäder, P., Kaiser, F., Adholeya, A., Singh, R., Uppal, H. S., Anil, K., et al. (2011). Inoculation of root microorganisms for sustainable wheat-rice and wheat-black gram rotations in India. Soil Biology & Biochemistry, 43, 609–619.

    Article  CAS  Google Scholar 

  • Mantelin, S., & Touraine, B. (2004). Plant growth-promoting bacteria and nitrate availability: Impacts on root development and nitrate uptake. Journal of Experimental Botany, 55, 27–34.

    Article  CAS  PubMed  Google Scholar 

  • Martins, B. E. (2015). Caracterização morfológica, bioquímica e molecular de isolados bacterianos antagonistas a Magnaporthe oryzae. 80 f. Dissertação, Universidade Federal de Goiás.

  • Muthukumarasamy, R., Cleenwerck, I., Revathi, G., Vadivelu, M., Janssens, D., Hoste, B., et al. (2005). Natural association of Gluconacetobacter diazotrophicus and diazotrophic Acetobacter peroxydans with wetland rice. Systematic and Applied Microbiology, 28, 277–286.

    Article  CAS  PubMed  Google Scholar 

  • Nascente, A. S., Filippi, M. C. C., Lanna, A. C., Souza, A. C. A., Silva-Lobo, V. L., & Silva, G. B. (2017a). Biomass, gas exchange, and nutrient contents in upland rice plants affected by application forms of microorganism growth promoters. Environmental Science and Pollution Research, 24, 2956–2965.

    Article  CAS  PubMed  Google Scholar 

  • Nascente, A. S., Filippi, M. C. C., Lanna, A. C., Souza, A. C. A., Silva-Lobo, V. L., & Silva, G. B. (2017b). Effects of beneficial microorganisms on lowland rice development. Environmental Science and Pollution Research, 24, 25233–25242.

    Article  PubMed  Google Scholar 

  • Olanrewaju, O. S., Glick, B. R., & Babalola, O. O. (2017). Mechanisms of action of plant growth promoting bacteria. World Journal of Microbiology & Biotechnology, 33, 197.

    Article  CAS  Google Scholar 

  • Ono, K., Maruyama, A., Kuwagata, T., Mano, M., Takimoto, T., Hayashi, K., et al. (2013). Canopy-scale relationships between stomatal conductance and photosynthesis in irrigated rice. Global Change Biology, 19, 2209–2220.

    Article  PubMed  Google Scholar 

  • Pérez-Montaño, F., Alías-Villegas, C., Bellogín, R. A., del Cerro, P., Espuny, M. R., Jiménez-Guerrero, I., et al. (2014). Plant growth promotion in cereal and leguminous agricultural important plants: From microorganism capacities to crop production. Microbiological Research, 169, 325–336.

    Article  PubMed  Google Scholar 

  • Rangel, P. H. H., Neto, F. P. M., Fagundes, P. R. R., de Magalhaes Jr, A. M., de Morais, O. P., Schmidt, A. B., et al. (2010). Development of herbicide-tolerant irrigated rice cultivars Pesq. Agropecu. Bras., 45, 701–708.

    Article  Google Scholar 

  • Reeves, T. G., Waddington, S. R., Ortiz-Monasterio, I., Bänziger, M., & Cassaday, K. (2002). Removing nutritional limits to maize and wheat production: A developing country perspective. In I. R. Kennedy & A. Choudhury (Eds.), Biofertilizers in action (pp. 11–36). Canberra: Research and Development Corporation.

    Google Scholar 

  • Rose, M. T., Phuong, T. L., Nhan, D. K., Cong, P. T., Hien, N. T., & Kennedy, I. R. (2014). Up to 52% N fertilizer replaced by biofertilizer in lowland rice via farmer participatory research. Agronomy for Sustainable Development, 34, 857–868.

    Article  Google Scholar 

  • Saharan, B. S., & Nehra, V. (2011). Plant-growth-promoting rhizobacteria: A critical review. Life Sciences and Medicine Research, 21, 1–30.

    Google Scholar 

  • SAS. (1999). Procedure guide for personal computers (Quinta ed.). Cary: SAS Institute.

    Google Scholar 

  • Shaharoona, B., Naveed, M., Arshad, M., & Zahir, Z. A. (2008). Fertilizer-dependent efficiency of Pseudomonads for improving growth, yield, and nutrient use efficiency of wheat (Triticum aestivum L.). Applied Microbiology and Biotechnology, 79, 147–155.

    Article  CAS  PubMed  Google Scholar 

  • Silva, R. O., Barioni, L. G., Zanett Albertini, T., Eory, V., Toop, C. F. E., Fernandes, F. A., et al. (2016). Developing a nationally appropriate mitigation measure from the greenhouse gas GHG abatement potential from livestock production in the Brazilian Cerrado. Agricultural Systems, 140, 48–55.

    Article  Google Scholar 

  • Sousa, D. M. G., & Lobato, E. (2004). Cerrado: correção do solo e adubação (segunda ed.). Brasília: Embrapa Cerrados.

    Google Scholar 

  • Souza, R., Ambrosini, A., & Passaglia, L. M. P. (2015). Plant growth-promoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology, 38(4), 401–419.

    Article  PubMed  PubMed Central  Google Scholar 

  • Spaepen, S., Vanderleyden, J., & Okon, Y. (2009). Plant growth-promoting actions of rhizobacteria. Advances in Botanical Research, 51, 284–320.

    Google Scholar 

  • Sperandio, E. M., Vale, H. M. M., Reis, M. S., Cortes, M. V. C. B., Lanna, A. C., & Filippi, M. C. C. (2017). Evaluation of rhizobacteria in upland rice in Brazil: Growth promotion and interaction of induced defense responses against leaf blast (Magnaporthe oryzae). Acta Physiologiae Plantarum, 39, 259.

    Article  CAS  Google Scholar 

  • Tajini, F., Trabelsi, M., & Drevon, J. J. (2012). Combined inoculation with Glomus intraradices and Rhizobium tropici CIAT899 increases phosphorus use efficiency for symbi-otic nitrogen fixation in common bean (Phaseolus vulgaris L.). Saudi Journal of Biological Sciences, 19, 157–163.

    Article  CAS  PubMed  Google Scholar 

  • Yao, L. X., Wu, Z. S., Zheng, Y. Y., Kaleem, I., & Li, C. (2010). Growth promotion and protection against salt stress by Pseudomonas putida Rs-198 on cotton. European Journal of Soil Biology, 46, 49–54.

    Article  CAS  Google Scholar 

  • Zahir, Z. A., Munir, A., Asghar, H. N., Shaharoona, B., & Arshad, M. (2018). Effectiveness of rhizobacteria containing ACC deaminase for growth promotion of pea (Pisum sativum) under drought conditions. Journal of Microbiology and Biotechnology, 18, 958–963.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Embrapa Arroz e Feijão, Rodovia GO-462, Km 12, Caixa postal 179, Santo Antônio de Goiás, GO, CEP 75375-000, Brazil

    Adriano Stephan Nascente, Anna Cristina Lanna & Marta Cristina Corsi de Filippi

  2. Escola de Agronomia, Universidade Federal de Goiás, Rodovia Goiânia/Nova Veneza km 0, Goiânia, GO, CEP 74001-970, Brazil

    Thatyane Pereira de Sousa

  3. Departamento de Fitopatologia, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF, CEP 70910-900, Brazil

    Amanda Abdallah Chaibub

  4. Departamento de Fitopatologia, Universidade Federal de Lavras, Campus Universitário, Lavras, MG, CEP 37200-000, Brazil

    Alan Carlos Alves de Souza

Authors
  1. Adriano Stephan Nascente
    View author publications

    Search author on:PubMed Google Scholar

  2. Anna Cristina Lanna
    View author publications

    Search author on:PubMed Google Scholar

  3. Thatyane Pereira de Sousa
    View author publications

    Search author on:PubMed Google Scholar

  4. Amanda Abdallah Chaibub
    View author publications

    Search author on:PubMed Google Scholar

  5. Alan Carlos Alves de Souza
    View author publications

    Search author on:PubMed Google Scholar

  6. Marta Cristina Corsi de Filippi
    View author publications

    Search author on:PubMed Google Scholar

Corresponding author

Correspondence to Marta Cristina Corsi de Filippi.

Ethics declarations

Conflict of interest

No content conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Nascente, A.S., Lanna, A.C., de Sousa, T.P. et al. N Fertilizer Dose-Dependent Efficiency of Serratia spp. for Improving Growth and Yield of Upland Rice (Oryza sativa L.). Int. J. Plant Prod. 13, 217–226 (2019). https://doi.org/10.1007/s42106-019-00049-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue date:

  • DOI: https://doi.org/10.1007/s42106-019-00049-5

Keywords