Hello, author! EVE is a very good job, Thank you so much for your contributions to the community.

I recently encountered some problems when I was using EVE to score genetic variation. I am looking forward to your reply very much!

I would like to ask the following three questions:

1）What are "_ASM" and "_BPU"? Is there a help document that describes information for each column? When the two results are different, which one should be chosen? For example, csv files for PTEN

2）What transcripts do the 3,000 + proteins on EVE's website refer to? Because I found that the different transcription, variation of the corresponding amino acid is different, I refer to is MAEN project (refer to the link: http://tark.ensembl.org/web/mane_project/)

The Matched Annotation from NCBI and EMBL-EBI (MANE) is a collaboration that aims to converge on human gene annotation and to produce a genome wide transcript set that includes pairs of RefSeq (NM) and Ensembl/GENCODE (ENST) transcripts that are 100% identical.

3）Why is EVE's score of a protein missing a large fragment? Like the HCN4 protein (https://evemodel.org/proteins/HCN4_HUMAN)

I am looking forward to your reply very much!

Kind regards,
Licko