diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 599fd53af..42f930fd0 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -37,13 +37,13 @@ jobs:
 
       - name: Build new docker image
         if: env.MATCHED_FILES
-        run: docker build --no-cache . -t nfcore/eager:dev
+        run: docker build --no-cache . -t nfcore/eager:2.4.3
 
       - name: Pull docker image
         if: ${{ !env.MATCHED_FILES }}
         run: |
           docker pull nfcore/eager:dev
-          docker tag nfcore/eager:dev nfcore/eager:dev
+          docker tag nfcore/eager:dev nfcore/eager:2.4.3
 
       - name: Install Nextflow
         env:
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 612a91836..d8e697845 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -3,7 +3,7 @@
 The format is based on [Keep a Changelog](http://keepachangelog.com/en/1.0.0/)
 and this project adheres to [Semantic Versioning](http://semver.org/spec/v2.0.0.html).
 
-## [dev]
+## [2.4.3] - 2022-03-24
 
 ### `Added`
 
diff --git a/Dockerfile b/Dockerfile
index 0938a51f1..865026492 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -7,7 +7,7 @@ COPY environment.yml /
 RUN conda env create --quiet -f /environment.yml && conda clean -a
 
 # Add conda installation dir to PATH (instead of doing 'conda activate')
-ENV PATH /opt/conda/envs/nf-core-eager-2.4.3dev/bin:$PATH
+ENV PATH /opt/conda/envs/nf-core-eager-2.4.3/bin:$PATH
 
 # Dump the details of the installed packages to a file for posterity
-RUN conda env export --name nf-core-eager-2.4.3dev > nf-core-eager-2.4.3dev.yml
+RUN conda env export --name nf-core-eager-2.4.3 > nf-core-eager-2.4.3.yml
\ No newline at end of file
diff --git a/environment.yml b/environment.yml
index 39d8ce233..293dadc6c 100644
--- a/environment.yml
+++ b/environment.yml
@@ -1,6 +1,6 @@
 # You can use this file to create a conda environment for this pipeline:
 #   conda env create -f environment.yml
-name: nf-core-eager-2.4.3dev
+name: nf-core-eager-2.4.3
 channels:
   - conda-forge
   - bioconda
@@ -49,4 +49,4 @@ dependencies:
   - bioconda::eigenstratdatabasetools=1.0.2
   - bioconda::mapdamage2=2.2.1
   - bioconda::bbmap=38.92
-  - bioconda::bcftools=1.12
+  - bioconda::bcftools=1.12
\ No newline at end of file
diff --git a/nextflow.config b/nextflow.config
index 282a26b81..09f31a367 100644
--- a/nextflow.config
+++ b/nextflow.config
@@ -284,7 +284,7 @@ params {
 
 // Container slug. Stable releases should specify release tag!
 // Developmental code should specify :dev
-process.container = 'nfcore/eager:dev'
+process.container = 'nfcore/eager:2.4.3'
 
 // Load base.config by default for all pipelines
 includeConfig 'conf/base.config'
@@ -414,7 +414,7 @@ manifest {
   description = 'A fully reproducible and state-of-the-art ancient DNA analysis pipeline'
   mainScript = 'main.nf'
   nextflowVersion = '>=20.07.1'
-  version = '2.4.3dev'
+  version = '2.4.3'
 }
 
 // Function to ensure that resource requirements don't go beyond