diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index fe0129e270..54945781c8 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -1,6 +1,6 @@
 repos:
   - repo: https://github.com/astral-sh/ruff-pre-commit
-    rev: v0.13.3
+    rev: v0.14.3
     hooks:
       - id: ruff-check # linter
         args: [--fix, --exit-non-zero-on-fix] # sort imports and fix
diff --git a/CHANGELOG.md b/CHANGELOG.md
index 05cb1e2d49..52ee30f079 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -6,6 +6,7 @@
 
 - add optional link to blogpost to sync PR ([#3852](https://github.com/nf-core/tools/pull/3852))
 - update multiqc version to fix utils test ([#3853](https://github.com/nf-core/tools/pull/3853))
+- Update pre-commit hook astral-sh/ruff-pre-commit to v0.14.3 ([#3861](https://github.com/nf-core/tools/pull/3861))
 
 ### Template