diff --git a/CHANGELOG.md b/CHANGELOG.md
index c9633bd0db..00dd4a6537 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -6,6 +6,7 @@
 
 - add optional link to blogpost to sync PR ([#3852](https://github.com/nf-core/tools/pull/3852))
 - update multiqc version to fix utils test ([#3853](https://github.com/nf-core/tools/pull/3853))
+- Update python:3.14-slim Docker digest to 4ed3310 ([#3862](https://github.com/nf-core/tools/pull/3862))
 - Update dependency textual-dev to v1.8.0 ([#3860](https://github.com/nf-core/tools/pull/3860))
 - Update pre-commit hook astral-sh/ruff-pre-commit to v0.14.3 ([#3861](https://github.com/nf-core/tools/pull/3861))
 
diff --git a/Dockerfile b/Dockerfile
index dc353175d6..25e67aff38 100644
--- a/Dockerfile
+++ b/Dockerfile
@@ -1,4 +1,4 @@
-FROM python:3.14-slim@sha256:1e7c3510ceb3d6ebb499c86e1c418b95cb4e5e2f682f8e195069f470135f8d51
+FROM python:3.14-slim@sha256:4ed33101ee7ec299041cc41dd268dae17031184be94384b1ce7936dc4e5dead3
 LABEL authors="phil.ewels@seqera.io,erik.danielsson@scilifelab.se" \
     description="Docker image containing requirements for nf-core/tools"