Imagine a variant in hg19 space:
chromosome = '21'
position = 36252851
ref = 'T'
alt = 'TAGG'
This is ambiguous with:
chromosome = '21'
position = 36252852
ref = 'A'
alt = 'AGGA'
According to the 3' rule, either of these would result in:
NC_000021.8:g.36252852_36252853insGGA
Translation to c. becomes:
NM_001754.5:c.508+1_508+2insTCC
We believe the actual change here should be the following, based on 3' realignment of the coding sequence, oriented on the negative strand:
NM_001754.5:c.508+2_508+3insCCT
Setting a g. nomenclature without realignment (36252851_36252852insAGG) yields the correct coding nomenclature.