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Description
Hi and thanks for the revival of this very useful tool! All other RNA-seq tools are quite difficult to use, with specialized inputs, so it is very helpful to have one so straightforward.
I had a question about your description of the Expression Profiling Efficiency. In the Metrics.md file, it is indicated
The proportion of "Exonic Reads" (see "Exonic Rate", below) out of all reads which were not Secondary Alignments or Platform/Vendor QC Failing reads.
This would mean that it is something like "Exonic Reads / Mapping reads". But this would be the same as the exonic rate.
Seeing that the Expression Profiling Efficiency is actually "Mapping Rate * Exonic Rate", it looks like it is more akin to "Exonic Reads / Total Reads", maybe a rewording of the description might be useful. When using MultiQC, the popup help actually says "Ratio of exonic reads to total reads".
Or maybe it is only I that is confused by the wording of the description!