Abstract
Different pigments often occur together and affect photosynthetic characteristics of the respective leaf portions. In this study, photosynthetic activity in variegated leaves of five cultivars of the ornamental and medicinal plant, Coleus × hybridus hort., was estimated by image analysis and point data measurements of major chlorophyll (Chl) fluorescence parameters and related to the amount of photosynthetic pigments measured with a Chl meter or spectrophotometrically in leaf extracts. Significant differences in Chl and carotenoid (Car) contents were noticed among differentially pigmented sectors of a leaf and among the cultivars. Although the higher Chl concentration was noticed in purple parts compared to green parts of the leaves, the values of minimal and maximal fluorescence yield at the dark- and light-adapted state (F0, Fm, F0', Fm', respectively) were a little lower than those in the green sectors, indicating photoprotective effects provided by anthocyanins and Car, more abundant in the red parts. The lowest Chl and Car content was detected in creamy-yellow and pink sectors and this contributed to low F0, Fm, and Fm', maximal quantum yield of PSII photochemistry, and nonphotochemical and photochemical quenching but high PSII maximum efficiency and effective quantum yield of PSII photochemistry. Both methods of Chl fluorescence analysis revealed heterogeneity in capture, transfer, and dissipation of excitation energy but Chl fluorescence imaging was more suitable in examining very narrow pigmented leaf areas.
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Abbreviations
- CA:
-
Camaroon
- Car(s):
-
carotenoid(s)
- CF:
-
chlorophyll fluorescence
- Chl:
-
chlorophyll
- DD:
-
Dappled Down
- F0, Fm :
-
minimal and maximal fluorescence yield at the dark-adapted state
- F0', Fm':
-
minimal and maximal fluorescence yield at the light-adapted state
- Ft :
-
steady-state fluorescence yield
- Fv :
-
variable fluorescence at the dark-adapted state
- Fv/Fm :
-
maximal quantum yield of PSII photochemistry
- FM :
-
fresh mass
- SD:
-
standard deviation
- NPQ:
-
Stern-Volmer nonphotochemical quenching coefficient
- NR:
-
Neon Rose
- qP:
-
photochemical quenching coefficient
- RU:
-
Ruby
- SR:
-
Sunlover Red Ruffles
- ΦPSII :
-
effective quantum yield of PSII photochemistry
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Acknowledgments: The study was supported by the University of Agriculture in Krakow, DS 3113.
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Borek, M., Bączek-Kwinta, R. & Rapacz, M. Photosynthetic activity of variegated leaves of Coleus × hybridus hort. cultivars characterised by chlorophyll fluorescence techniques. Photosynthetica 54, 331–339 (2016). https://doi.org/10.1007/s11099-016-0225-7
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DOI: https://doi.org/10.1007/s11099-016-0225-7