Abstract
Wholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zebrafish that can be adapted to use on embryos from the single-cell stage until 5 days postfertilization.
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Mathiyalagan, N., Dworkin, S. (2022). Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development. In: Dworkin, S. (eds) Craniofacial Development. Methods in Molecular Biology, vol 2403. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1847-9_2
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DOI: https://doi.org/10.1007/978-1-0716-1847-9_2
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