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Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development

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Craniofacial Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2403))

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Abstract

Wholemount in-situ hybridization in zebrafish is a powerful technique for visualizing spatiotemporal gene expression during development. Here we describe a technique to detect endogenous mRNA expression in zebrafish that can be adapted to use on embryos from the single-cell stage until 5 days postfertilization.

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Correspondence to Sebastian Dworkin .

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Mathiyalagan, N., Dworkin, S. (2022). Wholemount In-Situ Hybridization (WISH) in Zebrafish Embryos to Analyze Craniofacial Development. In: Dworkin, S. (eds) Craniofacial Development. Methods in Molecular Biology, vol 2403. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1847-9_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1847-9_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1846-2

  • Online ISBN: 978-1-0716-1847-9

  • eBook Packages: Springer Protocols

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