Fig. 2

Expression of tdTomato in the olfactory bulb (OB) of adult Drd2 reporter rats. a Diagram of rat brain sagittal section (top) and coronal section (bottom). The dashed line of the sagittal section diagram indicates the position of the coronal section. b Expression of tdTomato in OB. Scale bar, 400 µm. c Immunofluorescent image of tdTomato, OMP, and DAPI in the olfactory epithelial layer. Arrows indicate Drd2⁺ olfactory sensory neurons. Scale bar, 20 µm. d The percentage of Drd2⁺ cells in different layers among total Drd2⁺ cells in the OB. e Expression of tdTomato when injection of AAV-LSL-tdTomato into the GCL of adult Drd2::Cre rats. Scale bar, 500 µm. f Expression of tdTomato in GL. Scale bar, 200 µm. g Immunofluorescent image of tdTomato, TH, and GAD67 from the rectangle in f. Scale bar, 40 µm. Arrows indicate Drd2⁺ cells expressing both TH and GAD67, arrowheads indicate Drd2⁺ cells only expressing TH and empty arrows indicate Drd2⁺ cells expressing neither TH nor GAD67. h Immunofluorescent image of tdTomato and TBX 21 in the MCL. Scale bar, 25 µm. i Immunofluorescent image of tdTomato and GAD67 in the GCL. Scale bar, 25 µm. ONL olfactory nerve layer, GL glomerular layer, EPL external plexiform layer, MCL mitral cell layer, GCL granular cell layer, RMS rostral migratory stream