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Fig. 1 | Fisheries Science

Fig. 1

From: MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

Fig. 1

(From Miya et al. 2015 with slight modifications)

Schematic representation of the paired-end library preparation using a two-step tailed PCR for MiSeq sequencing ((MiSeq system, Illumina Inc.). a First-round PCR (1st PCR) to amplify a region of interest using a pair of MiFish primers (blue) with overhang adapter sequences (orange), b second-round PCR to add dual indices (red) and sequencing adapters (green).

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