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Fig. 8 | Fisheries Science

Fig. 8

From: MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

Fig. 8

(From Miya et al. 2015, with slight modifications)

Neighbor-joining trees of the seven tuna species based on the amplified regions using MiFish-U (12S rRNA gene) and MiFish-tuna (ND5 gene) primer pairs. The two trees were constructed using MEGA7 (Kumar et al. 2016). The two tuna species contained in the aquarium tank (yellowfin and Pacific bluefin) are highlighted in bold. Distances were calculated using the Kimura’s two-parameter model of base substitution with gaps being deleted. Numbers beside the internal branches are bootstrap probabilities based on 300 pseudo-replicates, and branch lengths are proportional to substitutions per site.

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