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Figure 4 | Scientific Reports

Figure 4

From: Molecular developmental mechanism in polypterid fish provides insight into the origin of vertebrate lungs

Figure 4

Functional assay for the core lung mesenchyme-specific enhancer of the Tbx4 gene.

Various vectors were introduced into the right-hand side of the chicken lung mesenchyme: (a–d) the ptk-EGFP vector alone as a control, (e–h) psTbx4C-LME4× and (i–l) lcTbx4C-LME4×. pCAGGS-Kusabira-orange was co-introduced to monitor the plasmid-positive area (b,f,j). The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (a,e,i). No expression was observed in the control (c), whereas enhanced green fluorescent protein (EGFP) expression from psTbx4C-LME4× and lcTbx4C-LME4× was detected in the lung mesenchyme (g,k). The signals from Kusabira-orange were merged with the EGFP expression (d,h,l). Asterisks indicate the right lung bud. Scale bars, 200 μm.

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