Fig. 6

PEBP gene expression in C. sativa. (a) Heatmap of RNA-seq data performed on flowering and vegetative tissue samples from ‘FINOLA’, ‘Felina 32’ and F₂ individuals, mapped to ‘Kompolti’ haplotype A and B. Each row is an average TPM of the biological replicates. Each sample type has 3-5 biological replicates. Growth stages refer to the number of true leaf pairs [55], while ‘days’ refers to days post sowing [8]. Note that, although all transcripts were mapped to both Kompolti haplotypes, the female and monoecious plants have no Y chromosome. Transcripts from female or monoecious plants mapping to Y chromosomal genes probably originated from X chromosomal FT genes. Likewise, ‘Felina 32’ encodes CsFT1 and ‘FINOLA’ encodes both CsFT1a and CsFT1b. (b) A comparison of the CsFT2 expression in female (stage 2, L2F) and male (stage 2, L2M) shoot apical meristem samples (cv. ‘FINOLA’) before flowering. RNA-seq data was mapped to both Kompolti haplotypes separately for the male transcripts. Although this doesn’t allow distinguishing whether the transcripts originated from the X or Y chromosome it is clear from the statistics that CsFT2a transcripts are overexpressed in males as compared to females. Kruskal–Wallis with Conover-Iman post-hoc test was used, α = 0.05 with letters denoting significance levels.