Abstract
This chapter explores the application of trichomorphology for taxonomical purposes. Throughout the book, the focus is on guard hairs, which are the most universally used and diagnostically significant type of hairs in taxonomy—i.e. they possess the most comprehensive diagnostic criteria. Once hairs are sampled, they must be meticulously prepared for microscopic examination. Detailed descriptions are provided for the physical and chemical methods required to prepare hairs for effective examination of the medulla and cuticle. These methods include the preparation of microscope slides, techniques for preparing and using gelatin to obtain clear cuticle imprints, the application of specific types of turpentine to clarify the medulla, and, in extreme cases of completely darkened medullas, the use of peroxide to discolour hairs. This chapter concludes with a discussion on the various morphological criteria to be examined once the preparation of the microscope slides is completed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mills DJ, Harris B, Claridge AW, Barry SC. Efficacy of hair-sampling techniques for the detection of medium-sized terrestrial mammals. I. A comparison between hair-funnels, hair-tubes and indirect signs. Wildl Res. 2002;29(4):379–87. https://doi.org/10.1071/WR01031.
Bremner-Harrison S, Harrison SWR, Cypher BL, Murdoch JD, Maldonado J, Darden SK. Development of a single-sampling noninvasive hair snare. Wildl Soc Bull. 2006;34(2):456–61. https://doi.org/10.2193/0091-7648(2006)34[456:DOASNH]2.0.CO;2.
Balmori-de la Puente A, Escoda L, Fernández-González Á, Menéndez-Pérez D, González-Esteban J, Castresana J. Evaluating the use of non-invasive hair sampling and ddRAD to characterize populations of endangered species: application to a peripheral population of the European mink. Ecol Evol. 2023;13(9):e10530. https://doi.org/10.1002/ece3.10530.
Moore JE. A key for the identification of animal hairs. J Forensic Sci Soc. 1988;28(5):335–9. https://doi.org/10.1016/S0015-7368(88)72862-5.
Knecht L. Chapter 8 – the use of hair morphology in the identification of mammals. In: Wildlife forensics: methods and applications. Oxford: John Wiley & Sons, Ltd.; 2012. p. 129–44.
Linacre A. Forensic science in wildlife investigations. Boca Raton: CRC Press; 2009. p. 178.
Menotti-Raymond MA, David VA, O’Brien SJ. Pet cat hair implicates murder suspect. Nature. 1997;386(6627):774. https://doi.org/10.1038/386774a0.
Tridico SR, Houck MM, Kirkbride KP, Smith ME, Yates BC. Morphological identification of animal hairs: myths and misconceptions, possibilities and pitfalls. Forensic Sci Int. 2014;238:101–7. https://doi.org/10.1016/j.forsciint.2014.02.023.
Mendiburt GB, Agostini V, Betancourt GC. Morphological differentiation of bovine and equine hair for species’ identification in forensic veterinary investigations. Forensic Sci Int. 2021;328:111037. https://doi.org/10.1016/j.forsciint.2021.111037.
Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis. Nat Methods. 2012;9(7):671–5. https://doi.org/10.1038/nmeth.2089.
Project I. Inkscape [Internet]. 2020. https://inkscape.org.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2025 Canadian Museum of Nature
About this chapter
Cite this chapter
Fontaine-Topaloff, J., Fauteux, D., Khidas, K. (2025). Towards Applied Trichomorphology. In: Hair Atlas of Canadian Mammals. Springer, Cham. https://doi.org/10.1007/978-3-032-00006-4_3
Download citation
DOI: https://doi.org/10.1007/978-3-032-00006-4_3
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-032-00005-7
Online ISBN: 978-3-032-00006-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)