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Part of the book series: Elsevier Applied Biotechnology Series ((APBISE))

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Abstract

Dunaliella is a unicellular, biflagellate, naked green alga (Chlorophyceae, Dunaliellales), and the type species of this genus, Dunaliella salina (Dunal) Teodoresco is often found in natural hypersaline waters where it colours the brines red (Teodoresco, 1905). This algal species was first recognised as containing high intracellular concentrations of β-carotene by Mil’ko (1963) and Aasen et al (1969). Initial research on the potential of using this alga as a commercial source of β-carotene began in the Ukraine in the 1960s (cf. Massyuk, 1966; Massyuk & Abdula, 1969) and it was later also proposed as a commercial source of glycerol (Ben-Amotz, 1980; Chen & Chi, 1981; Ben-Amotz & Avron, 1982).

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References

  • Aasen, A. J., Eimhjellen, K. E. & Liaaen-Jensen, S. (1969). An extreme source of β-carotene, Acta Chim. Scand., 23, 2544–5.

    Article  CAS  Google Scholar 

  • Avron, M., Ben-Amotz, A. & Edelstein, S.(1987). Feed supplement. UK patent application 2 189 675A.

    Google Scholar 

  • Bauernfeind, J. C. (ed.) (1981). Carotenoids as Colourants and Vitamin A Precursors. Academic Press, New York, pp. 938.

    Google Scholar 

  • Ben-Amotz, A. (1980). Glycerol production in the alga Dunaliella. In Biochemical and Photosynthetic Aspects of Energy Production, ed. A. San Pietro. Academic Press, New York, pp. 191–208.

    Google Scholar 

  • Ben-Amotz, A. & Avron, M. (1982). The potential use of Dunaliella for the production of glycerol, β-carotene and high protein feed. In Biosaline Research: A Look to the Future, ed. A. San Pietro. Plenum Publishing Corporation, New York, pp. 207–14.

    Google Scholar 

  • Ben-Amotz, A. & Avron, M.(1983). On the factors which determine the massive β-carotene accumulation in the halotolerant alga Dunaliella salina. Plant Physiol.,72, 593–7.

    Article  CAS  Google Scholar 

  • Ben-Amotz, A., Katz, A. & Avron, M. (1982). Accumulation of β-carotene inhalotolerant algae: purification and characterisation of β-carotene-rich globules from Dunaliella bardawil (Chlorophyceae). J. Phycol., 18, 529–37.

    Article  CAS  Google Scholar 

  • Ben-Amotz, A., Edelstein, S. & Avron, M. (1986). Use of the β-carotene rich alga Dunaliella bardawil as a source of retinol. Br. Poult. Sci., 27, 613–9.

    Article  CAS  Google Scholar 

  • Bloch, M. R., Sasson, J., Ginzburg, M. E., Goldman, Z., Ginzburg, B. Z., Garti, N. & Perath, A. (1982). Oil products from algae. US patent no. 4.341 038.

    Google Scholar 

  • Borowitzka, L. J. (1981). The microflora. Adaptions to life in extremely saline lakes. Hydrobiologia, 81, 33–46.

    Google Scholar 

  • Borowitzka, M. A.(1988). Algal growth media and sources of algal cultures. In Micro-algal Biotechnology, ed. M. A. Borowitzka & L. J. Borowitzka, Cambridge University Press, Cambridge pp. 456–65.

    Google Scholar 

  • Borowitzka,L. J., Borowitzka, M. A. & Moulton, T. P.(1984). The mass culture of Dunaliella salina for fine chemicals: from laboratory to pilot plant. Hydrobiologia, 116/117, 115–21.

    Article  Google Scholar 

  • Borowitzka, L. J., Moulton, T. P. & Borowitzka, M. A. (1986). Salinity and the commercial production of beta-carotene from Dunaliella salina. Nova Hedwigia, Beih., 83, 224–9.

    Google Scholar 

  • Borowitzka, M. A. & Borowitzka, L. J. (1988a) Dunaliella. In Microalgal Biotechnology, ed. M. A. Borowitzka & L. J. Borowitzka. Cambridge University Press, Cambridge, pp. 27–58.

    Google Scholar 

  • Borowitzka, M. A. & Borowitzka, L. J.(1988b) Limits to growth and carotenogenesis in laboratory and large-scale cultures of Dunaliella salina. In Algal Biotechnology, ed. T. Stadler, J. Mollion, M-C. Verdus, Y. Karamanos, H. Morvan & D. Christiaen. Elsevier Applied Science Publishers, Barking, pp. 371–82.

    Google Scholar 

  • Chen, B. J. & Chi, C. H. (1981). Process development and evaluation for algal glycerol production. Biotech. Bioeng., 23, 1267–87.

    Article  CAS  Google Scholar 

  • Craig,R., Reichelt, B. Y. & Reichelt, J. L.(1988).Genetic engineering of micro-algae. In Micro-algal Biotechnology, ed. M.A. Borowitzka & L. J. Borowitzka. Cambridge University Press, Cambridge, pp. 415–55.

    Google Scholar 

  • Curtain, C. C. & Snook, H. (1983). Method for harvesting algae. US patent no. 511 135.

    Google Scholar 

  • Curtain, C. C., West, S. M. & Schlipalius, L. (1987). Manufacture of β-carotene from the salt lake alga Dunaliella salina; the scientific and technical background. Aust. J. Biotechnol., 1, 51–7.

    CAS  Google Scholar 

  • Drokova, I. G. & Dovhorouka, S. I.(1966). Carotene-formation in Dunaliella salina Teod. under the effect of some carbon sources. Ukransk. Bot. Zhour., 21, 59–62.

    Google Scholar 

  • Federov, V. D., Maksimov, V. N. & Kromov, V. M. (1968). Effect of light and temperature on primary production of certain unicellular green algae and diatoms. Fiziol. Rast., 15, 640–51.

    Google Scholar 

  • Gibor, A. (1956). The culture of brine algae. Biol, bull., Woods Hole, 3, 223–9.

    Article  Google Scholar 

  • Grant, B. R. (1968). The effect of carbon dioxide concentration and buffer system onnitrate and nitrite and assimilation by Dunaliella tertiolecta, J. Gen. Micro., 54, 327–36.

    CAS  Google Scholar 

  • Kessler, J. O. (1982). Algal cell harvesting. US patent no. 4 324 067.

    Google Scholar 

  • Kessler, J. O. (1985). Hydrodynamic focusing of motile algal cells. Nature, 313, 208–10.

    Article  Google Scholar 

  • Kläui,H (1981).Industrial and commercial uses of carotenoids. In Carotenoid chemistry and Biochemistry, ed. G. Britton & T. W. Goodwin. Pergamon Press, Oxford, pp. 309–28.

    Google Scholar 

  • Klausner, A. (1986). Algaculture: Food for thought. Biotechnology, 4, 947–53.

    Article  Google Scholar 

  • Lerche, W. (1937). Untersuchungen über die Entwicklung und Fortpflanzung in der Gattung Dunaliella. Arch, für Protistenk., 88, 236–9.

    CAS  Google Scholar 

  • Loeblich, L. A. (1972). Studies on the brine flagellate Dunaliella salina. PhD thesis, University of California, San Diego.

    Google Scholar 

  • MacKinney, G. & Chichester, C. O.(1960).Biosynthesis of carotenoids. In Comparative Biochemistry of Photoreactive Systems, ed. M. B. Allen. Academic Press, New York, pp. 205–15.

    Google Scholar 

  • Massyuk, N. P. (1965). Carbonate and bicarbonate as stimulators of growth and carotene accumulation in Dunaliella salina Teod. Ukransk. Bot. Zhour., 22, 18–22.

    Google Scholar 

  • Massyuk, N. P. (1966). Mass culture of the carotene-bearing alga Dunaliella salina Teod. Ukransk. Bot. Zhour., 23, 12–19.

    Google Scholar 

  • Massyuk, N. P. & Abdula, E. G. (1969). First experiment of growing carotene-containing algae under semi-industrial conditions. Ukransk. Bot. Zhour., 26, 21–7.

    Google Scholar 

  • Mathews-Roth, M. M.(1982).Medical applications and uses of carotenoids. In Carotenoid Chemistry and Biochemistry, ed. G. Britton & T. W. Goodwin. Pergamon Press, Oxford, pp. 297–307

    Google Scholar 

  • Mayer,H.& Isler, O. (1971). Total syntheses. In Carotenoids, ed. O. Isler. Birkhauser, Basle, pp. 328–575.

    Google Scholar 

  • Mil’ko, E. S. (1962). Study the requirement of two Dunaliella spp. in mineral and organic components if the medium, Moscow University Vestnik.Biologya,6, 21–3.

    Google Scholar 

  • Mil’ko, E. S. (1963). Effect of various environmental factors on pigment production in the alga Dunaliella salina. Mikrobiologiya, 32, 299–307.

    Google Scholar 

  • Mironyuk, V. I. & Einor, L. O.(1968). Oxygen exchange and pigment content in various forms of Dunaliella salina Teod. under conditions of increasing NaCl content. Gidrobiol Zhournal, 4, 23–9.

    CAS  Google Scholar 

  • Mohn, F. H. (1988). Harvesting of micro-algal biomass. In Micro-algal Biotechnology, ed. M. A. Borowitzka & L. J. Borowitzka. Cambridge University Press, pp. 395–414.

    Google Scholar 

  • Moulton, T. P., Borowitzka, L. J. & Vincent, D. J. (1987a) the mass culture of Dunaliella salina for β-carotene: from pilot plant to production plant, Hydrobiologia, 151/152, 99–105.

    Article  Google Scholar 

  • Moulton, T. P., Sommer, T. R., Burford, M. A. & Borowitzka, L. J. (1987b). Competition between Dunaliella species at high salinity. Hydrobiologia, 151/152, 107–16.

    Article  Google Scholar 

  • Nelis, H. J. C. F. & De Leenheer, A. P. (1983). Isocratic nonaqueous reversed-phase liquid chromatography of carotenoids. Analyt. Chem., 55, 270–75.

    Article  CAS  Google Scholar 

  • Nonomura, A. M. (1987). Process for producing a naturally derived carotene-oil composition by direct extraction from algae. US patent no. 4,680,314.

    Google Scholar 

  • Potts, W. T. (1987). Extraction of carotenoid pigments from algae. Australian patent application no. 69260/87. Rich, V. (1978). Israel’s place in the sun. Nature, 275, 581-2.

    Google Scholar 

  • Rich, V.(1978). Israel’s place in the sun. Nature, 275, 581–2.

    Article  Google Scholar 

  • Ruane, M. (1974a). Recovery of algae from brine suspensions. Australian patent no. 486 999.

    Google Scholar 

  • Ruane, M. (1974b). Extraction of caroteiniferous materials from algae. Australian patent no. 487 018.

    Google Scholar 

  • Sammy, N. (1987). Method for harvesting algae. Australian patent application no. 70924/87.

    Google Scholar 

  • Schwartz, J., Suda, D. & Light, G. (1986). Beta-carotene is associated with the regression of hamster buccal puch carcinoma and induction of tumor necrosis factor in macrophages. Biochem. biophys. Res. Commun., 136, 1130–5.

    Article  CAS  Google Scholar 

  • Semenko, V. E. & Abdullayev, A. A. (1980). Parametric control of ß-carotene biosynthesis in Dunaliella salina cells under conditions of intensive cultivation. Fiziol. Rast., 27, 31–41.

    Google Scholar 

  • Siegel, B. Z., Siegel, S. M., Speitel, T., Waber, J. & Stoeker, R. (1984). Brine organisms and the question of habitat-specific adaption. Origins of Life, 14, 757–70.

    Article  CAS  Google Scholar 

  • Spencer, J. F. T. & Spencer, D. M. (1983). Genetic improvements of industrial yeasts. Ann. Rev. Microbiol., 37, 121–42.

    Article  CAS  Google Scholar 

  • Teodoresco, E. C.1905). Organisation et developpement du Dunaliella nouveau genre de Volvocacee-Polyblepharidee. Bot. Zentralblatt, Beih., 18, 215–32.

    Google Scholar 

  • Wegmann, K., Ben-Amotz, A. & Avron, M. (1980). Effect of temperature on glycerol retention in the halotolerant algae Dunaliella and Asteromonas. Plant. Physiol., 66, 1196–7.

    Article  CAS  Google Scholar 

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© 1989 Elsevier Science Publishers Ltd

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Borowitzka, L.J., Borowitzka, M.A. (1989). β-Carotene (Provitamin A) Production with Algae. In: Vandamme, E.J. (eds) Biotechnology of Vitamins, Pigments and Growth Factors. Elsevier Applied Biotechnology Series. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1111-6_2

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  • DOI: https://doi.org/10.1007/978-94-009-1111-6_2

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-6991-5

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