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Molecular cloning of the Arabidopsis thaliana sedoheptulose-1,7-biphosphatase gene and expression studies in wheat and Arabidopsis thaliana

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Abstract

We report here the isolation and nucleotide sequence of genomic clones encoding the chloroplast enzyme sedoheptulose-1,7-bisphosphatase (SBPase) from Arabidopsis thaliana. The coding region of this gene contains eight exons (72–76 bp) and seven introns (75–91 bp) and encodes a polypeptide of 393 amino acids. Unusually, the 5′ non-coding region contains two additional AUG codons upstream of the translation initiation codon. A comparison of the deduced Arabidopsis and wheat SBPase polypeptide sequences reveals 78.6%, identity. Expression studies showed that the level of SBPase mRNA in Arabidopsis and wheat is regulated in a light-dependent manner and is also influenced by the developmental stage of the leaf. Although the Arabidopsis SBPase gene is present in a single copy, two hybridizing transcripts were detected in some tissues, suggesting the presence of alternate transcription start sites in the upstream region.

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Authors and Affiliations

  1. Biology Department, University of Essex, Wivenhoe Park, CO4 3SQ, Colchester, UK

    Nicola M. Willingham, Julie C. Lloyd & Christine A. Raines

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  1. Nicola M. Willingham
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  2. Julie C. Lloyd
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  3. Christine A. Raines
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Willingham, N.M., Lloyd, J.C. & Raines, C.A. Molecular cloning of the Arabidopsis thaliana sedoheptulose-1,7-biphosphatase gene and expression studies in wheat and Arabidopsis thaliana . Plant Mol Biol 26, 1191–1200 (1994). https://doi.org/10.1007/BF00040699

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  • DOI: https://doi.org/10.1007/BF00040699

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