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Selection of yeast able to produce ethanol from glucose at 40° C

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Summary

A total of 55 yeast strains selected from 7 genera known to ferment carbohydrates to ethanol were screened for their ability to ferment glucose to ethanol in shaken flask culture at 37°, 40° and 45°C. Yields of more than 50% of the theoretical maximum were obtained with 28 strains at 37°C, but only 12 at 40°C. Only 6 could grow at 45°C, but they produced poor yields. In general Kluyveromyces strains were more thermotolerant than Saccharomyces and Candida strains, but Saccharomyces strains produced higher ethanol yields. The 8 strains with the highest yields at 40°C were evaluated in batch fermentations. Three of these, two Saccharomyces and one Candida, were able to meet minimum commercial targets set at 8% (v/v) ethanol from 14% (w/v) glucose at 40°C.

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Hacking, A.J., Taylor, I.W.F. & Hanas, C.M. Selection of yeast able to produce ethanol from glucose at 40° C. Appl Microbiol Biotechnol 19, 361–363 (1984). https://doi.org/10.1007/BF00253786

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  • DOI: https://doi.org/10.1007/BF00253786

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