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QTL mapping of ten agronomic traits on the soybean (Glycine max L. Merr.) genetic map and their association with EST markers

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Abstract

A set of 184 recombinant inbred lines (RILs) derived from soybean vars. Kefeng No.1 × Nannong 1138-2 was used to construct a genetic linkage map. The two parents exhibit contrasting characteristics for most of the traits that were mapped. Using restricted fragment length polymorphisms (RFLPs), simple sequence repeats (SSRs) and expressed sequence tags (ESTs), we mapped 452 markers onto 21 linkage groups and covered 3,595.9 cM of the soybean genome. All of the linkage groups except linkage group F were consistent with those of the consensus map of Cregan et al. (1999). Linkage group F was divided into two linkage groups, F1 and F2. The map consisted of 189 RFLPs, 219 SSRs, 40 ESTs, three R gene loci and one phenotype marker. Ten agronomic traits—days to flowering, days to maturity, plant height, number of nodes on main stem, lodging, number of pods per node, protein content, oil content, 100-seed weight, and plot yield—were studied. Using winqtlcart, we detected 63 quantitative trait loci (QTLs) that had LOD>3 for nine of the agronomic traits (only exception being seed oil content) and mapped these on 12 linkage groups. Most of the QTLs were clustered, especially on groups B1 and C2. Some QTLs were mapped to the same loci. This pleiotropism was common for most of the QTLs, and one QTL could influence at most five traits. Seven EST markers were found to be linked closely with or located at the same loci as the QTLs. EST marker GmKF059a, encoding a repressor protein and mapped on group C2, accounted for about 20% of the total variation of days to flowering, plant height, lodging and nodes on the main stem, respectively.

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Acknowledgements

This work was supported by National 973 project (G1998010209 and 2002CB111301) and National High Technology Program (2002AA211051).

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Institute of Genetics and Developmental Biology, The Chinese Academy of Sciences, 100101, Beijing, China

    W.-K. Zhang, Y.-J. Wang, G.-Z. Luo, J.-S. Zhang, C.-Y. He, X.-L. Wu & S.-Y. Chen

  2. Soybean Research Institute of Nanjing Agricultural University, National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, 210095, Nanjing , China

    J.-Y. Gai

Authors
  1. W.-K. Zhang
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  2. Y.-J. Wang
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  3. G.-Z. Luo
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  4. J.-S. Zhang
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  5. C.-Y. He
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  6. X.-L. Wu
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  7. J.-Y. Gai
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  8. S.-Y. Chen
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Corresponding authors

Correspondence to J.-Y. Gai or S.-Y. Chen.

Additional information

Communicated by H.F. Linskens

W.-K. Zhang, Y.-J. Wang and G.-Z. Luo contributed equally to this investigation.

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Zhang, WK., Wang, YJ., Luo, GZ. et al. QTL mapping of ten agronomic traits on the soybean (Glycine max L. Merr.) genetic map and their association with EST markers. Theor Appl Genet 108, 1131–1139 (2004). https://doi.org/10.1007/s00122-003-1527-2

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  • DOI: https://doi.org/10.1007/s00122-003-1527-2

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