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A Novel EPS-Producing Strain of Bacillus licheniformis Isolated from a Shallow Vent Off Panarea Island (Italy)

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Abstract

A haloalkaliphilic, thermophilic Bacillus strain (T14), isolated from a shallow hydrothermal vent of Panarea Island (Italy), produced a new exopolysaccharide (EPS). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain T14 was highly related (99 % similarity) to Bacillus licheniformis DSM 13T and Bacillus sonorensis DSM 13779T. Further DNA–DNA hybridization analysis revealed 79.40 % similarity with B. licheniformis DSM 13T and 39.12 % with B. sonorensis DSM 13779T. Sucrose (5 %) was the most efficient carbon source for growth and EPS production. The highest EPS production (366 mg l−1) was yielded in fermenter culture at 300 rpm after 48 h of incubation. The purified fraction EPS1 contained fructose/fucose/glucose/galactosamine/mannose in a relative proportion of 1.0:0.75:0.28:tr:tr and possessed a molecular weight of 1,000 kDa displaying a trisaccharide unit constituted by sugars with a β-manno-pyranosidic configuration. Screening for biological activity showed anti-cytotoxic effect of EPS1 against Avarol in brine shrimp test, indicating a potential use in the development of novel drugs.

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Authors and Affiliations

  1. Department of Biological and Environmental Sciences, University of Messina, V.le F. Stagno d’Alcontres 31, 98166, Messina, Italy

    Antonio Spanò, Concetta Gugliandolo, Valeria Lentini & Teresa L. Maugeri

  2. C.N.R. Institute of Biomolecular Chemistry I.C.B., Via Campi Flegrei 34, 80078, Pozzuoli, Naples, Italy

    Gianluca Anzelmo, Annarita Poli & Barbara Nicolaus

Authors
  1. Antonio Spanò
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  2. Concetta Gugliandolo
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  3. Valeria Lentini
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  4. Teresa L. Maugeri
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  5. Gianluca Anzelmo
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  6. Annarita Poli
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  7. Barbara Nicolaus
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Correspondence to Concetta Gugliandolo.

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Spanò, A., Gugliandolo, C., Lentini, V. et al. A Novel EPS-Producing Strain of Bacillus licheniformis Isolated from a Shallow Vent Off Panarea Island (Italy). Curr Microbiol 67, 21–29 (2013). https://doi.org/10.1007/s00284-013-0327-4

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