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Alleviation of salt stress of symbiotic Galega officinalis L. (goat's rue) by co-inoculation of Rhizobium with root-colonizing Pseudomonas

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Abstract

Background and aims

Salt stress is an increasing problem in agricultural soils in many parts of the world, and salt tolerant cropping systems are in great demand. We investigated the effect of co-inoculation of Galega officinalis with Rhizobium galegae and two plant growth promoting Pseudomonas species on plant growth, nodulation, and N content under salt stress.

Methods

The effect of inoculation with R. galegae sv. officinalis HAMBI 1141 alone and in combination with the root-colonizing Pseudomonas extremorientalis TSAU20 or Pseudomonas trivialis 3Re27 on the growth of G. officinalis exposed to salt stress (50 and 75 mM NaCl) was studied under gnotobiotic and greenhouse conditions.

Results

The growth of goat’s rue was reduced at 50 and 75 mM NaCl both in the gnotobiotic sand system and in low-fertilized potting soil in the greenhouse. Co-inoculation of unstressed and salt-stressed goat’s rue with R. galegae HAMBI 1141 and either P. extremorientalis TSAU20 or P. trivialis 3Re27 significantly improved root and shoot growth and increased nodulation of plant roots in both growth systems compared with plants inoculated with R. galegae alone. The nitrogen content of co-inoculated plant roots was significantly increased at 75 mM NaCl in potting soil. Co-inoculation of G. officinalis with either of the two plant growth promoting (PGPR) Pseudomonas strains also improved root tip-colonization by R. galegae cells.

Conclusions

The co-inoculation of goat’s rue with Rhizobium and PGPR Pseudomonas strains alleviated salt stress of plants grown in NaCl-amended gnotobiotic sand systems and in potting soil in the greenhouse.

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Acknowledgments

This study was supported by the UNESCO-L”OREAL Fellowship for “Women in Science” and the Academy of Finland. We thank Li Li (University of Helsinki) for technical assistance in the greenhouse and Marjut Wallner (University of Helsinki) for the analysis of the plant nitrogen content.

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Authors and Affiliations

  1. Faculty of Biology and Soil Sciences, National University of Uzbekistan, Tashkent, 100174, Uzbekistan

    Dilfuza Egamberdieva

  2. Institute of Environmental Biotechnology, Graz University of Technology, Graz, Austria

    Gabriela Berg

  3. Department of Food and Environmental Sciences, University of Helsinki, P.O. Box 56, Biocenter 1, Viikinkaari 9, Helsinki, 00014, Finland

    Dilfuza Egamberdieva, Kristina Lindström & Leena A. Räsänen

  4. Department of Agronomical Microbiology, Institute of Soil Science and Plant Cultivation, State Research Institute, Czartoryskich 8, Puławy, 24100, Poland

    Leena A. Räsänen

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  1. Dilfuza Egamberdieva
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Correspondence to Dilfuza Egamberdieva.

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Egamberdieva, D., Berg, G., Lindström, K. et al. Alleviation of salt stress of symbiotic Galega officinalis L. (goat's rue) by co-inoculation of Rhizobium with root-colonizing Pseudomonas . Plant Soil 369, 453–465 (2013). https://doi.org/10.1007/s11104-013-1586-3

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