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Isolation and Characterization of Low Molecular Weight Glycosaminoglycans from Marine Mollusc Amussium pleuronectus (Linne) using Chromatography

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Abstract

The glycosaminoglycan (GAG) heparin is a polyanionic sulfated polysaccharide most recognized for its anticoagulant activity. In the present study, the GAGs were extracted from bivalve mollusc Amussium pleuronectus. The crude GAGs were fractionated by ion-exchange (DEAE-cellulose and Amberlite IRA-900 & 120) chromatography. The recovered active fractions (as determined by metachromatic assay) were confirmed by agarose gel electrophoresis and the active fractions were purified in Sephadex G-100 column. Fractionated and purified GAG molecular weight was determined through gradient polyacrylamide gel electrophoresis. The structural characterization of low molecular weight GAG was analyzed by Fourier transform infrared spectroscopy. The activated partial thromboplastin time of purified GAG is 95 IU/mg and has molecular weight 6,500–7,500 Da. The disaccharide compositional analysis on the GAG sample was sulfated like porcine intestinal mucosal heparan sulfate, and it contains equivalent amount of uronic acid and hexosamine. The results of this study suggest that the GAG from A. pleuronectus could be an alternative source of heparin.

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Acknowledgement

The authors are thankful to the authorities of Annamalai University and the Director, CAS in Marine Biology for providing the facilities to carry out this work. One of the authors (RS) is also thankful to the ICMR for the financial assistance in the form of SRF.

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  1. Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai-608 502, Annamalai Nagar, Tamil Nadu, India

    R. Saravanan & A. Shanmugam

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  1. R. Saravanan
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  2. A. Shanmugam
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Correspondence to R. Saravanan.

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Saravanan, R., Shanmugam, A. Isolation and Characterization of Low Molecular Weight Glycosaminoglycans from Marine Mollusc Amussium pleuronectus (Linne) using Chromatography. Appl Biochem Biotechnol 160, 791–799 (2010). https://doi.org/10.1007/s12010-008-8498-3

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