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Production of Oligosaccharide from Alginate Using Pseudoalteromonas agarovorans

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Abstract

A marine bacterium was isolated from seawater near the Korean south coast for efficient saccharification from alginate. Based on 16S rDNA sequence, the isolated strain was identified as Pseudoalteromonas agarovorans. Various environmental factors affecting saccharification of alginate using P. agarovorans CHO-12 have been investigated in flask cultures. The optimum concentration of sugar was obtained at 30 rpm and 29 °C. Among various NaCl concentrations, when NaCl concentration was increased from 10 to 30 g/l, the cell concentration sharply increased, while there is no increase at above 40 g/l. The maximum sugar concentration was obtained at 13.8 when 30 g/l of NaCl was used. Yeast extract and corn steep liquor were the best nitrogen source for efficient saccharification. Especially, the sugar concentration of 14.9 g/l was obtained after 3 days of culture using a mixture of 1.0 g/l of yeast extract and 1.5 g/l of corn steep liquor. Scale up was carried out at 50 l of reactor for 3 days using P. agarovorans CHO-12 and Stenotrophomonas maltophilia sp. When S. maltophilia was used, cell concentration was about twofold higher than that of P. agarovorans CHO-12. On the other hand, when P. agarovorans CHO-12 was used, the maximum saccharification rate was obtained, 7.5 g/l/day after 2 days of culture, which was about tenfold higher than that of S. maltophilia.

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Acknowledgement

This research was financially supported by Chosun University, 2007.

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Authors and Affiliations

  1. Department of Environmental Health, Cho-dang University, Chonnam, 534-800, South Korea

    DuBok Choi

  2. Department of Polymer Science and Engineering, Chosun University, Gwangju, 501-759, South Korea

    Yu Lan Piao & Hoon Cho

  3. Department of Microbiology, College of Medicine, Kwandong University, Gangneung, 210-701, South Korea

    Woon-Seob Shin

Authors
  1. DuBok Choi
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  2. Yu Lan Piao
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  3. Woon-Seob Shin
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  4. Hoon Cho
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Corresponding authors

Correspondence to Woon-Seob Shin or Hoon Cho.

Additional information

Woon-Seob Shin and Hoon Cho contributed equally to this work.

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Choi, D., Piao, Y.L., Shin, WS. et al. Production of Oligosaccharide from Alginate Using Pseudoalteromonas agarovorans . Appl Biochem Biotechnol 159, 438–452 (2009). https://doi.org/10.1007/s12010-008-8514-7

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  • DOI: https://doi.org/10.1007/s12010-008-8514-7

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