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Combining Genome Shuffling and Interspecific Hybridization Among Streptomyces Improved ε-Poly-l-Lysine Production

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Abstract

Here we first improved the ε-PL productivity in five species of wild-type strains in Streptomyces (Streptomyces padanus, Streptomyces griseofuscus, Streptomyces graminearus, Streptomyces hygroscopicus, and Streptomyces albulus) by genome shuffling. Then all the shuffled strains were suffered from an interspecific hybridization through stochastic protoplast fusion. One hybrid designated FEEL-1 was selected by morphology and spore color with ε-PL production of 1.12 g/L in shake flask, about 2.75-fold higher than that in wild types. The ε-PL production of FEEL-1 was then obtained as 24.5 g/L in fed-batch fermentation, which was 63–81 % higher than those in shuffled strains. Random amplified polymorphic DNA revealed that FEEL-1 was probably hybridized from S. padanus, S. griseofuscus, and S. albulus. Activities of several enzymes in FEEL-1 (hexokinase, phosphoenolpyruvate carboxylase, aspartokinase, and citrate synthase) were more active than those in shuffled strains, which was a possible reason for the enhancement of ε-PL production. This research highlights the importance of genome shuffling along with interspecific hybridization as a new breeding strategy for improving phenotype of industrial strains.

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Acknowledgments

This work was supported by a grant from Wuxi Science and Technology Support Program (CYE21N1107), the Priority Academic Program Development of Jiangsu Higher Education Institutions, and 111 Project (111-2-06).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, People’s Republic of China

    Shu Li, Lei Tang & Zhonggui Mao

  2. Xiwang Group Company Limited, Zouping, 256209, China

    Xusheng Chen & Chuanliang Dong

  3. Wuxi Century Biology Medicine Limited Company, Wuxi, 214092, China

    Fulin Zhao

Authors
  1. Shu Li
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  2. Xusheng Chen
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  3. Chuanliang Dong
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  4. Fulin Zhao
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  5. Lei Tang
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  6. Zhonggui Mao
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Correspondence to Lei Tang or Zhonggui Mao.

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Li, S., Chen, X., Dong, C. et al. Combining Genome Shuffling and Interspecific Hybridization Among Streptomyces Improved ε-Poly-l-Lysine Production. Appl Biochem Biotechnol 169, 338–350 (2013). https://doi.org/10.1007/s12010-012-9969-0

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  • DOI: https://doi.org/10.1007/s12010-012-9969-0

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