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Silver nanoparticles: characterization and antibacterial properties

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Abstract

In this study, two aqueous suspensions of silver nanoparticles, nano silver powder (Sigma Ag-NPs) and nano silver ink (Ink Ag-NPs), were characterized for studying their antibacterial activity. The X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM) were used to determine the crystal structure, shape and size of Ag-NPs. The bactericidal effects of these nanoparticles were investigated against gram positive and gram negative bacteria at two different concentrations, 10 and 50 \(\upmu \)g/disc, by the disk diffusion method using Mueller–Hinton Agar to display good microbial culture growth. Antibacterial experiments results revealed that Escherichia coli (ATCC25922) and Bacillus subtilis (ATCC6633) inhibited by Ink Ag-NPs more than Sigma Ag-NPs. Both Salmonella typhimurium (ATCC14028) and Staphylococcus aureus (ATCC25923) were resistant to both sources of Ag-NPs, while Pseudomonas aeruginosa (ATCC27853) was resistant to Ink Ag-NPs and inhibited by Sigma Ag-NPs. This experiment showed that characteristics and suspension stability of Ag-NPs affect its antibacterial activity against some pathogenic bacterial strains.

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Authors and Affiliations

  1. National Institute of Standards, P. O. Box 136, Giza, 12211, Egypt

    Rania Sayed

  2. Microbiology Section, Botany Department, Faculty of Science, Suez Canal University, Ismailia, 41522, Egypt

    Heba Saad & Nashwa Hagagy

Authors
  1. Rania Sayed
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  2. Heba Saad
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  3. Nashwa Hagagy
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Correspondence to Rania Sayed.

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Sayed, R., Saad, H. & Hagagy, N. Silver nanoparticles: characterization and antibacterial properties. Rend. Fis. Acc. Lincei 29, 81–86 (2018). https://doi.org/10.1007/s12210-017-0663-6

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  • DOI: https://doi.org/10.1007/s12210-017-0663-6

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