Diagnostic Performance of Metagenomic Next-Generation Sequencing (mNGS) and Culture in Infected Pancreatic Necrosis: A Systematic Review and Meta-Analysis Aizaz AliAsad Iqbal KhattakEmad Mansoor in Digestive Diseases and Sciences Article Open access 18 October 2025
Empowering low-crosstalk, dynamic-decision random access of DNA storage via 384-multiplexed nanopore signatures Junyao LiXuyang ZhaoYi Li in Nature Communications Article Open access 17 October 2025
Validating a remote saliva collection tool for genomic analyses in free ranging dogs Giulia CimarelliMartina LazzaroniMałgorzata Pilot in Scientific Reports Article Open access 17 October 2025
Novel multiplex family-wide PCR and Nanopore sequencing of amplicons (FP-NSA) approach for surveillance of influenza- and coronaviruses in humans and animals Irene Kasindi MekiKi Bum AhnCharles Euloge Lamien in Genome Medicine Article Open access 17 October 2025
Predicting sequence-specific amplification efficiency in multi-template PCR with deep learning Andreas L. GimpelBowen FanRobert N. Grass in Nature Communications Article Open access 16 October 2025
An optimized DNA extraction protocol for reliable PCR-based detection and characterization of grapevine flavescence dorée phytoplasma Marco CarliAthos PedrelliDomenico Rizzo in Plant Methods Article Open access 16 October 2025
Accurate somatic small variant discovery for multiple sequencing technologies with DeepSomatic Jimin ParkDaniel E. CookKishwar Shafin in Nature Biotechnology Article 16 October 2025
Improving somatic variant calling with long-read technologies and public datasets in Nature Biotechnology Article 16 October 2025
gReLU: a comprehensive framework for DNA sequence modeling and design Avantika LalLaura GunsalusGokcen Eraslan in Nature Methods Article Open access 15 October 2025
Analysis of cfDNA fragmentomics metrics and commercial targeted sequencing panels Kyle T. HelzerMarina N. SharifiShuang G. Zhao in Nature Communications Article Open access 14 October 2025
Genomic diversity and surveillance of SARS-CoV-2 in Nigeria Thomas J. Y. KonoEzenwa J. OnyemataKennedy Otwombe in BMC Genomics Article Open access 14 October 2025
Ending diagnostic odyssey by reanalysis of whole exome sequencing data: reclassification of suspected Fanconi anemia cases to dyskeratosis congenita and Diamond-Blackfan anemia Eudald TejeroMaría José Ramírez de HaroJordi Surrallés in Orphanet Journal of Rare Diseases Article Open access 14 October 2025
SAGPEK: fast and flexible approach to identify genotypes of Sanger sequencing data Jinpeng WangShuo SunJinming Huang in BMC Bioinformatics Article Open access 14 October 2025
Clinical long-read metagenomic sequencing of culture-negative infective endocarditis reveals genomic features and antimicrobial resistance Worarat KruasuwanDitthawat PathomchareansukchaiSupathep Tansirichaiya in BMC Infectious Diseases Article Open access 14 October 2025
Improving STR typing for low template DNA by abasic-site-mediated semi-linear preamplification Qiqi JiZhimin LiJianhui Xie in Scientific Reports Article Open access 13 October 2025
Whole-genome sequencing of Lassa virus from dry blood spots: a comparative evaluation Umaru BanguraChristopher DavisElisabeth Fichet-Calvet in Infectious Diseases of Poverty Article Open access 13 October 2025
Prior knowledge on context-driven DNA fragmentation probabilities can improve de novo genome assembly algorithms Patrick PflughauptAleksandr B. Sahakyan in BMC Bioinformatics Article Open access 13 October 2025
The potential of mRNA markers in body fluids and personal source analysis based on the QNome nanopore sequencing Suyu LiHaowen SongZheng Wang in International Journal of Legal Medicine Article 13 October 2025
Harnessing eDNA metabarcoding for mosquito surveillance: a pilot study in Ethiopia’s Great Rift Valley Samson LetaTesfaye Rufael ChibssaJan Paeshuyse in Molecular Biology Reports Article 11 October 2025
Validation of a dual-purpose conventional PCR strategy for SARS-CoV-2 detection and lineage screening in resource-limited laboratories Liliana Iñiguez-GutiérrezMaría Teresa Magaña-TorresTeresita de Jesús Hernández-Flores in Molecular Biology Reports Article 11 October 2025