Genetic Engineering

Latest research

1. Efficient production of gene-edited onion (Allium cepa) plants using biolistic delivery of cas9 RNPs and transient expression constructs

   • Cameron L. De La Mora
   • Michael J. Havey
   • Patrick J. Krysan

   in Plant Cell Reports

   Article Open access 19 October 2025

   

2. Targeted disruption of OsBADH2 induces basmati aroma in the popular indica rice variety IR-64 using CRISPR/Cas9

   • Vacha Bhatt
   • Thakku R. Ramkumar
   • Altafhusain Nadaf

   in Plant Physiology Reports

   Article 18 October 2025

   

3. Electroporation-based CRISPR/Cas9 Gene Editing in Haliotis Discus Hannai

   • Chen Liu
   • Dianzhi Yao
   • Xiaotong Wang

   in Marine Biotechnology

   Article 18 October 2025

   

4. Engineering the Cas9 nuclease to enhance prime editing precision

   • Alexandra Despang

   in Nature Biotechnology

   Article 14 October 2025

5. Programmable promoter editing for precise control of transgene expression

   • Sneha R. Kabaria
   • Yunbeen Bae
   • Kate E. Galloway

   in Nature Biotechnology

   Article 13 October 2025

   

6. Promoter editing generates stable setpoints of gene expression

   in Nature Biotechnology

   Article 13 October 2025

   

7. Should genetically modified wildlife be banned? Scientists weigh the risks

   • Mariana Lenharo

   in Nature

   Article 10 October 2025

   

8. ProPE expands the prime editing window and enhances gene editing efficiency where prime editing is inefficient

   • Sarah Laura Krausz
   • Dorottya Anna Simon
   • Ervin Welker

   in Nature Catalysis

   Article Open access 10 October 2025

   

9. Engineering gene drive docking sites in a haplolethal locus in Anopheles gambiae

   • Andrea L. Smidler
   • Eryney A. Marrogi
   • Kevin Esvelt

   in Scientific Reports

   Article Open access 08 October 2025

   

10. Genome-edited farm animals from haploid stem cells

    • Björn Oback

    in Nature Biotechnology

    Article 07 October 2025

    

11. Development of an improved Agrobacterium-mediated transformation and callus-based regeneration system for sugarbeet (Beta vulgaris L.)

    • Zainul Khan
    • Tinley Hathaway
    • Vanitharani Ramachandran

    in Plant Cell, Tissue and Organ Culture (PCTOC)

    Article Open access 07 October 2025

    

12. Generating Broad-Spectrum Resistance to ALS-Inhibiting Herbicides in Rice by CRISPR/Cas9-Mediated NHEJ

    • Chao Ouyang
    • Xiongxia Jin
    • Baoguang An

    in Rice

    Article Open access 03 October 2025

    

13. Genome editing for trait optimization: the role of CRISPR, base editing and prime editing in modern era of plant science

    • Varadharajan Nareshkumar
    • S. R. Harish Chandar
    • Chinnaswamy Appunu

    in Journal of Plant Biochemistry and Biotechnology

    Article 03 October 2025

    

14. Digital PCR-Based characterization of a Zhonghuang 6106 soybean genomic DNA reference material for its food and feed detection

    • Cheng Wang
    • Ruihuan Li
    • Xin Zhao

    in Scientific Reports

    Article Open access 03 October 2025

    

15. Exonuclease-fused CRISPR-cas system enhances targeted genome editing for functional genomics in soybean

    • Vikas Devkar
    • Kaushik Ghose
    • Gunvant B. Patil

    in BMC Plant Biology

    Article Open access 02 October 2025

    

16. Mini-review: prospects of in vitro mutagenesis for genetic improvement of Vanilla

    • José Luis Spinoso‑Castillo
    • Obdulia Baltazar-Bernal

    in Plant Cell, Tissue and Organ Culture (PCTOC)

    Article 29 September 2025

    

17. Lethal endotoxin (ccdB) based counterselection improved the efficiency of sequential gene editing in Escherichia coli

    • Shiyao Zou
    • Weiqi Chen
    • Shengde Zhou

    in Biotechnology Letters

    Article 29 September 2025

    

18. Exploring experts’ uncertainties about gene drive technology for agricultural pest control in the U.S.: a qualitative study to inform innovation and decision-making

    • Nourou Barry
    • S. Kathleen Barnhill
    • Branden B. Johnson

    in Environment Systems and Decisions

    Article Open access 26 September 2025

19. Spatio-temporal regulation of recombinase expression enables efficient autoexcision of selectable marker genes in soybean and maize

    • Brent O’Brien
    • Anagha Sant
    • Matthew S. Marengo

    in BMC Plant Biology

    Article Open access 24 September 2025

    

20. Engineered enzymes can suppress genome-editing errors

    in Nature

    Article 24 September 2025

    

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