@@ -31,11 +31,7 @@ Command:
3131 trim-5 trims 5' end of the reads from a FASTQ file.
3232 trim-3 trims 3' end of the reads from a FASTQ file.
3333 trim-id trims reads ids (removes everything after first space) from a FASTQ file.
34- trim-N trims Ns at both ends of the reads from a FASTQ file.
35- trim-polyA trims polyA at both ends of the reads from a FASTQ file.
36- trim-polyT trims polyT at both ends of the reads from a FASTQ file.
37- trim-polyC trims polyC at both ends of the reads from a FASTQ file.
38- trim-polyG trims polyG at both ends of the reads from a FASTQ file.
34+ trim-poly trims poly-A/C/G/T/N tails at both ends of the reads sequences from a FASTQ file.
3935 drop-se drops unpaired reads from an interleaved paired-end FASTQ file.
4036 drop-short drops reads that have short sequences (below a given threshold).
4137 fq2fa converts a FASTQ file to FASTA file.
@@ -44,6 +40,7 @@ Command:
4440 NtoA replaces all Ns in reads sequences with As in a FASTQ file.
4541 rev-com reverse complements all reads in a FASTQ file.
4642
43+
4744```
4845
4946Examples
@@ -109,11 +106,11 @@ Examples
109106
110107* Trim N or Ns from both ends of the reads sequences from FASTQ file:
111108
112- fastqtk trim-N reads.fq out.fq
109+ fastqtk trim-poly N 1 reads.fq out.fq
113110
114111* Trim polyA, which are strictly or equally longer than 15bp, from both ends of the reads sequences from FASTQ file:
115112
116- fastqtk trim-polyA 15 reads.fq out.fq
113+ fastqtk trim-poly A 15 reads.fq out.fq
117114
118115* Compress the reads ids from a FASTQ file:
119116
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