diff --git a/.github/workflows/jit.yml b/.github/workflows/jit.yml
index 754f179f105591..6469d88b25197a 100644
--- a/.github/workflows/jit.yml
+++ b/.github/workflows/jit.yml
@@ -5,6 +5,7 @@ on:
       - '**jit**'
       - 'Python/bytecodes.c'
       - 'Python/optimizer*.c'
+      - 'Python/pylifecycle.c'
       - '!Python/perf_jit_trampoline.c'
       - '!**/*.md'
       - '!**/*.ini'
@@ -13,6 +14,7 @@ on:
       - '**jit**'
       - 'Python/bytecodes.c'
       - 'Python/optimizer*.c'
+      - 'Python/pylifecycle.c'
       - '!Python/perf_jit_trampoline.c'
       - '!**/*.md'
       - '!**/*.ini'
diff --git a/Lib/test/test_embed.py b/Lib/test/test_embed.py
index 4ea43edccda63e..39bcac6ea3a6f5 100644
--- a/Lib/test/test_embed.py
+++ b/Lib/test/test_embed.py
@@ -1780,8 +1780,13 @@ def test_initconfig_api(self):
             'perf_profiling': 2,
         }
         config_dev_mode(preconfig, config)
+        is_using_jit = "_Py_JIT" in (sysconfig.get_config_var('PY_CORE_CFLAGS') or '')
+        if is_using_jit:
+            stderr = "<sys>:0: RuntimeWarning: JIT deactivated as perf profiling support is active"
+        else:
+            stderr = ""
         self.check_all_configs("test_initconfig_api", config, preconfig,
-                               api=API_ISOLATED)
+                               api=API_ISOLATED, env={'PYTHON_JIT': '0'}, stderr=stderr)
 
     def test_initconfig_get_api(self):
         self.run_embedded_interpreter("test_initconfig_get_api")
diff --git a/Lib/test/test_perf_profiler.py b/Lib/test/test_perf_profiler.py
index b55d441759eb69..1e74990878007a 100644
--- a/Lib/test/test_perf_profiler.py
+++ b/Lib/test/test_perf_profiler.py
@@ -210,14 +210,14 @@ def test_sys_api_with_existing_trampoline(self):
                 sys.activate_stack_trampoline("perf")
                 sys.activate_stack_trampoline("perf")
                 """
-        assert_python_ok("-c", code)
+        assert_python_ok("-c", code, PYTHON_JIT="0")
 
     def test_sys_api_with_invalid_trampoline(self):
         code = """if 1:
                 import sys
                 sys.activate_stack_trampoline("invalid")
                 """
-        rc, out, err = assert_python_failure("-c", code)
+        rc, out, err = assert_python_failure("-c", code, PYTHON_JIT="0")
         self.assertIn("invalid backend: invalid", err.decode())
 
     def test_sys_api_get_status(self):
@@ -228,7 +228,7 @@ def test_sys_api_get_status(self):
                 sys.deactivate_stack_trampoline()
                 assert sys.is_stack_trampoline_active() is False
                 """
-        assert_python_ok("-c", code)
+        assert_python_ok("-c", code, PYTHON_JIT="0")
 
 
 def is_unwinding_reliable_with_frame_pointers():
diff --git a/Python/pylifecycle.c b/Python/pylifecycle.c
index 2efaa9db7d7d58..31ccd0db768e1e 100644
--- a/Python/pylifecycle.c
+++ b/Python/pylifecycle.c
@@ -1310,12 +1310,15 @@ init_interp_main(PyThreadState *tstate)
             enabled = *env != '0';
         }
         if (enabled) {
+#ifdef _Py_JIT
             if (config->perf_profiling > 0) {
                 (void)PyErr_WarnEx(
                     PyExc_RuntimeWarning,
                     "JIT deactivated as perf profiling support is active",
                     0);
-            } else {
+            } else
+#endif
+            {
                 PyObject *opt = _PyOptimizer_NewUOpOptimizer();
                 if (opt == NULL) {
                     return _PyStatus_ERR("can't initialize optimizer");
diff --git a/Python/sysmodule.c b/Python/sysmodule.c
index cbb73977e1aae6..a4abd7c3c45709 100644
--- a/Python/sysmodule.c
+++ b/Python/sysmodule.c
@@ -2290,6 +2290,7 @@ sys_activate_stack_trampoline_impl(PyObject *module, const char *backend)
 #ifdef _Py_JIT
     _PyOptimizerObject* optimizer = _Py_GetOptimizer();
     if (optimizer != NULL) {
+        Py_DECREF(optimizer);
         PyErr_SetString(PyExc_ValueError, "Cannot activate the perf trampoline if the JIT is active");
         return NULL;
     }