diff --git a/changes/unreleased/4855.8hCFRFMeMaRWpBEYaxrTMq.toml b/changes/unreleased/4855.8hCFRFMeMaRWpBEYaxrTMq.toml
new file mode 100644
index 00000000000..cbbda59a270
--- /dev/null
+++ b/changes/unreleased/4855.8hCFRFMeMaRWpBEYaxrTMq.toml
@@ -0,0 +1,5 @@
+other= "Make Gender Input Case-Insensitive in ``conversationbot.py``"
+[[pull_requests]]
+uid = "4855"
+author_uid = "fengxiaohu"
+closes_threads = ["4846"]
diff --git a/examples/conversationbot.py b/examples/conversationbot.py
index 751f48f74e0..dbe637c1203 100644
--- a/examples/conversationbot.py
+++ b/examples/conversationbot.py
@@ -145,7 +145,9 @@ def main() -> None:
     conv_handler = ConversationHandler(
         entry_points=[CommandHandler("start", start)],
         states={
-            GENDER: [MessageHandler(filters.Regex("^(Boy|Girl|Other)$"), gender)],
+            # Use case-insensitive regex to accept gender input regardless of letter casing,
+            # e.g., "boy", "BOY", "Girl", etc., will all be matched
+            GENDER: [MessageHandler(filters.Regex("(?i)^(Boy|Girl|Other)$"), gender)],
             PHOTO: [MessageHandler(filters.PHOTO, photo), CommandHandler("skip", skip_photo)],
             LOCATION: [
                 MessageHandler(filters.LOCATION, location),