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Size Exclusion Chromatography

Size exclusion chromatography (SEC), also known as gel permeation chromatography (GPC), separates molecules of different sizes using porous particles. Smaller molecules can enter the pores of the particles, taking a longer path and time to elute, while larger molecules that cannot enter the pores elute faster together. SEC is used to determine molecular weights and distributions of polymers by how molecules of different sizes have different transit times passing through the column.

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Size Exclusion Chromatography

Size exclusion chromatography (SEC), also known as gel permeation chromatography (GPC), separates molecules of different sizes using porous particles. Smaller molecules can enter the pores of the particles, taking a longer path and time to elute, while larger molecules that cannot enter the pores elute faster together. SEC is used to determine molecular weights and distributions of polymers by how molecules of different sizes have different transit times passing through the column.

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Size Exclusion Chromatography (SEC)

Introduction

Size-exculsion chromatography (SEC), also called gel-permeation chromatography


(GPC), uses porous particles to separate molecules of different sizes. It is generally used
to separate biological molecules and to determine molecular weights and molecular
weight distributions of polymers. Molecules that are smaller than the pore size can enter
the particles and therefore have a longer path and longer transist time than larger
molecules that cannot enter the particles.

Schematic of a size-exclusion chromatography column

All molecules larger than the pore size are unretained and elute together. Molecules that
can enter the pores will have an average residence time in the particles that depends on
the molecules size and shape. Different molecules therefore have different total transit
times through the column.

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