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Determining Glucose in Blood

This document outlines the process for determining glucose levels in blood samples. It involves: 1) Preparing a blood sample by adding reagents and centrifuging to remove proteins. 2) Determining glucose content by adding the sample to copper alkalis, boiling, cooling, adding arsenomolybdat, and measuring absorbance at 660nm. 3) Creating a standard curve by measuring absorbance of glucose solutions at different concentrations and using it to calculate glucose levels in samples.

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Anggraini Nugroho P
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19 views2 pages

Determining Glucose in Blood

This document outlines the process for determining glucose levels in blood samples. It involves: 1) Preparing a blood sample by adding reagents and centrifuging to remove proteins. 2) Determining glucose content by adding the sample to copper alkalis, boiling, cooling, adding arsenomolybdat, and measuring absorbance at 660nm. 3) Creating a standard curve by measuring absorbance of glucose solutions at different concentrations and using it to calculate glucose levels in samples.

Uploaded by

Anggraini Nugroho P
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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Download as DOC, PDF, TXT or read online on Scribd
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Determining Glucose in blood

1. Preparing sampel
1 mL blood sample
Poured into test tube
Stirred
added with 1 mL Ba(OH)2 0,3 N
Mixed with mL ZnSO4.7H2O 5%
Mixed
Added2 mL (NH4)2SO4 0,5 N
Stayed for 15 minutes
Centrifuged 15 minutes in 3500 rpm
Decantace

Filtrate residue
taken 1 mL
added 5 drops burette
changing color
sample zero protein

2. Determining glucose content in blood


1 mL blood sample zero protein
Poured into test tube
Added 3 mL Cu alkalis
Inserted in boiling water for 15 minutes
Inserted in cooled water 10 minutes
Added 3-5 drops 2 drops arsenomolybdat
Mixed
Read adsorbance with spectrotonic 20 in wave 660 nm

Adsorbancy

Making of standard Curve

1 mL glucose 1 mL glucose 1 mL glucose 1 mL glucose


[0,3 mg/ml] [0,5 mg/ml] [0,7 mg/ml] [0,9 mg/ml]

poured into test tube


Added 3 mL Cu alkalis
Inserted into boiling water 15 minutes
Inserted into cooled water for 10 minutes
Added 2 drops arsenmollibdat
Read adsorbance with spektrotonic 20 in wave 660 nm

Adsorbancy
Blanko solution

1 mL aquades
Poured into test tube
Added 3 mL Cu alkalis
Inserted into boiling water 15 minutes
Inserted into cooled water for 10 minutes
Added 2 drops arsenmollibdat
Stirred
Read adsorbance with spektrotonic 20 in wave 660 nm

Adsorbancy

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