Equipment Requirements based on ASBC Methods
Beer 1 – Sampling
Beer 2 – Specific Gravity
Beer 2A – By Pycnometer (Archived)
Beer 2B – By Digital Density Meter -
Digital Density Meter (Anton Paar is recommended) - Mettler TOLEDO Da-110m
Portable Digital Density Specific Gravity Meter, $800 (eBay). Mettler TOLEDO Da-110
Density/specific Gravity Meter, $850,
Hypodermic syringe or instrument autosampler (Anton Paar)
Beer 3 – Apparent Extract
Beer 3A – By Digital Density Meter (See above)
Beer 3B – By Hydrometer for Beer
Hydrometer+cylinder - $20
Water Bath
Distilled Water
Beer
Wort
Beer 4 – Alcohol
Beer 4A – Beer and Distillate Measured Volumetrically
Distilling Flask, 500ml
Entrainment Trap, Kjeldahl-type (spherical/cylindrical)
Vertical condenser, Graham, Liebig, Allihn type 400mm long
Volumetric Flask, 100ml
Digital Density Meter
Hypodermic Syringe
Delivery pipet, 100ml
Beer 4B – Beer and Distillate Measured Gravimetrically
Balance of 500g and weights
Tared distilling flask, 500ml
Entrainment Trap / Vertical Condenser
Digital Density Meter / Hypo Syringes
Beer 4C – Alcohol Determined Refractometrically
Distilled water, 500ml to 1000ml daily
Immersion Refractometer, Carl Zeiss Code No. 50-02-00 w/prisms of 1.32-1.37
Light Source, Cuvet, Cuvet Carrier for refractometer
Water bath - PolyScience WB02A11B Digital General Purpose Water Bath $400-500
Digital Density Meter / Hypo Syringes
Hydrometer and Cylinder
Beer 4D – Ethanol Determined by Gas Chromatography
n-Propanol, internal standard, 5% v/v aqueous stock soln.
Ethanol, 5% v/v aqueous stock soln.
Gas Chromatograph with flame ionization detector (FID) and recorder – Agilent HP
6850GH FID $6000 (eBay) / Agilent 6890 N FID w/ Computer/software $16000 (eBay), HP GC w/FID &
NPD $1200 (eBay), Agilent 6890 single FID 6way valve, PC, $10,000 (eBay), aimanalytical.com
Chromatographic column: 6 ft x 1/8 in stainless steel or glass
Chromosorb 103, 80-100 mesh
Helium or Nitrogen
Hydrogen
Syringe, 1uL
Flasks, with stoppers
Pipets, appropriate sizes
S & S 560 filter paper
Beer 4E – Instrumental Method for Alcohol and Original Gravity
Ethyl Alcohol 95% v/v
Detergent, Tecator, cat no. 5000-1928
Ethanol
Tecator SCABA Automatic Beer Analyzer
Flasks, 1 L
Graduated cylinder, 100/200mL
Vials, Tecator, cat no. 5000-1822
Beer 4F – Enzymatic method for Low Alcohol Concentrations
Boehringer Mannheim GmbH ethanol (UV-method) cat no. 176 290)
Refridgerator for 4C
Redistilled or distilled deionized water
Sodium Hydroxide soln 12.5%
Spectrophotometer 340nm
Cuvets, 1cm, 4mL
Volumetric pipets, 3mL, 10mL
Micropipets, 0.1mL and 0.04mL
Volumetric flasks, 100mL
Erlenmeyer flasks, 250mL
Beer 4G – Near-Infrared and Original Extract Content
Ethyl Alcohol 95% v/v
Distilled Water
Anton Paar Alcolyzer/Density Meter beer analyzer
Sample vials, 35mL nylon caps Anton Paar cat no. 6776
Volumetric flask, 100mL
Pipette, 9.2mL
Funnel, Nalgene, top ID 158mm, stem top OD 14mm, stem length 151mm
Filter paper, Ahlstrom grade 509, 32cm diameter
Container, 500mL Nalgene w/cap
Sonication Bath
Beer 5 – Real Extract
Beer 5A – Beer Measured Volumetrically
Volumetric flask, 100mL
Digital Density Meter/hypo syringes
Beer 5B – Beer Measured Gravimetrically
Digital Density Meter/hypo syringes
Beer 5C – Real Extract Determined Refractometrically
Distilled water
Immersion refractometer
Water bath
Digital Density Meter/hypo syringes
Beer 8 – Total Acidity
Beer 8A – By Titration using Digital pH Meter
Buffer, pH 4, 7, 10
NaOH, 0.1M, CAS no. 1310-73-2
Commercial pH Meter (+/- 0.02pH units
Beaker, 100mL
Stir plate/stir bar
Buret, 25mL to 50mL
Pipette, 50mL
Beer 8B – By Titration using Phenolphthalein as Indicator
Phenolphthalein soln, 0.5% in 95% ethyl alchol, CAS no. 77-09-8
Standard NaOH, 0.1N, CAS no. 1310-73-2
Beaker/Erlenmeyer flask, 500mL
Pipet, 25mL, fast-flowing
Buret
Beer 9 – pH
Buffer, pH 4.0, 7.0
PH Meter,
Thermometer,
Beakers, 100mL
Beer 10 – Color
Beer 10A – Spectrophotometric Color Method
Reagent water (18M-ohm resistivity or greater)
Precision spectrophotometer, bandwidth of 1nm or less at 430nm.
Cuvettes, 10mm or ½ inch
Beer 10B – Photometric Method
Any "filter photometer/abridges spectrophotometer," with 430nm filter, transmitting in
420-450nm region
Cell size with absorbance between 0.187 and 0.6999 (20-65%)
Beer 10C – Tristimulus Analysis
Reagent water (18M-ohm resistivity or greater)
Trsitimulus transmission colorimeter/precision spectophotometer
Sample cell/cuvettes, 10mm
Tristimulus analysis calculator (ASBC website)
Beer 11 – Protein
Beer 11A – By Kjeldahl
Reagent water (18M-ohm resist. CAS no. 7789-20-0
Sulfuric Acid, 0.05M, CAS no. 7664-93-9
Hydrochloric Acid, 0.1M, CAS no. 7647-01-0
Sodium hydroxide, 0.1M, CAS no. 1310-73-2
Sulfuric acid, 18M, nitrogen-free, CAS 7664-93-9
Titanium dioxide poweder, CAS 73463-67-7
Potassium sulfate, nitrogen-free, CAS 778-80-5
Copper sulfate pentahydrate, CAS 7758-99-8
Sodium hydroxide pellets, nitrate-free, CAS 1310-73-2
Sodium hydroxide soln, 45% w/v
Zinc granules, CAS 7440-66-6
Methyl red indicator, CAS 7440-66-6
Sucrose, CAS 57-50-1
Kjeldahl digestion rack, trapping H2SO4 fumes
Kjeldahl distillation unit w/ connecting bulb traps/vertical condensers
Kjeldahl flasks, 500mL or 800mL
Erlenmeyer flasks, 500mL
Pipette, 25mL
Burets, 50mL
Stoppererd/capped glass flask, 200mL
Balance, 500g +/-1.0g
Beer 11B – By Combustion
Lysine hydrocholoride, nitrogen standard, CAS 657-27-2
Reagent water (18M-ohm), CAS 7789-20-0
Oxygen, ultrapure grade
Helium, nitrogen-free
Combustion nitrogen analyzer w/thermal conductivity detector, reducing NOx to N2
Analytical balance, 0.1mg accuracy
Beer 11C – By Spectrophotometer
Reagent water, (18M-ohm), CAS 7789-20-0
Volumetric pipette, 6mL
Erlenmeyer flasks, 500mL, 1000mL
Top-loading balance, 1kg capacity, 0.01g resolution
Pasteur pipettes, 9in
Spectrophotometric cells, 1cm, quartz
UV/Vis spectrophotometer (single/dual beam), wavelengths as short as 215nm
Beer 13 – Dissolved Carbon Dioxide
Beer 13A – Pressure Method for Beer in Tanks
Zahm-Hartung CO2 Volume Meter
Beer 13B – Pressure Method for Beer in Bottles and Cans
Sodium hydroxide soln, 15%
Piercing apparatus, Zahm and Nagel
Absorption buret
Leveling bottle, 300mL
Water bath
Torsion balance/trip scale, 500g-1000g capacity, 0.1g sens
Graduated Cylinder, 100mL
Thermometer
Beer 13C – Manometric/Volumetric Method
Octyl Alcohol
Syringe, graduated with cap, 60cm^3
Parafilm
Barometer, able to read lb/in
Beer 13D – Volume Expansion Method
Water, grade 3
Beverage carbonation meter, Anton Paar CarboQC
Piercing and Filling Device (PFD), Anton Paar PFD
Graduated cylinder, 250mL
Absolute pressure sensor, 10 +/- 0.01 bar
Beer 14 – Percent Ash Determination
Evaporating dish, 100mL, platinum, fused silica, or porcelain
Hot plate/drying oven
Muffle furnace
Transfer pipette, 50mL
Analytical Balance, 0.1mg sens
Beer 16 – End Fermentation/Yeast Fermentable Extract
Compressed Brewers' yeast
Fermentation flasks, Erlenmeyer, 500mL, 1000mL
Fermentation seals, water-filled
Buchner fullnel
Filter paper
Balance, 0.1g sens
Graduated cylinder, 250mL
Rotary shaker, or stir plate/stir bar
Beer 18 – Iron
Beer 18A – Analysis by Colorimetry
Reagent water (18M-ohm)
Glacial Acetic Acid, CAS 64-19-7
Color Reagent, 2,2'-bipyridine CAS 366-18-7/orthophnanthroline CAS 66-71-7
Iron standard solution, 1000mg/L
Nitric Acid, CAS 7697-37-2
Ascorbic acid, CAS 50-81-7
Spectrophotometer/colorimeter
Water bath, 70C
Pipettes, volumetric, 1, 2, 5, 10, 20, 25, 30, 50mL
Volumetric flasks, 100, 500, 1000mL + stoppers
Cuvettes, 10mm glass, quartz, plastic
Balance, 0.001g res
Thermometer, accurate at 70C
Beer 18B – Analysis by Atomic Absorption Spectophotometry
Reagent water
Iron standard soln, 1000mg/L
Atomic absorption spectrophotometer, w/iron hollow cathode lamp
Volumetric flasks, glass-stoppered, 100mL
Pipettes, 1mL, 0.1mL graduated
Pipettes, 10mL, volumetric
Beer 20 – Calcium
Beer 20A – Chrome Black T indicator method
Various reagents
Various lab glassware and filters
Beer 20B – Calcein indicator Method
Various reagents
Various lab glassware
Beer 20C – Atomic absorption spectrophotometry
Various reagents
Atomic absorption spectrophotometer, w/ calcium hollow-cathode lamp
Various lab glassware
Beer 21 – Total Sulfure dioxide
Beer 21A – p-Rosaniline method
Various reagents
Photoelectric photometer, colorimeter, or spectrophotometer
Various lab glassware
Beer 21B – Segmented flow analyzer method
Various reagents
Segmented flow analyzer w/colorimetric detector, 560nm
Various lab glassware
Beer 22 – Foam Collapse Rate
Beer 22A – Sigma value method
Various lab glassware
Beer 22B – Foam flashing method
Isopropyl alcohol
Compresses CO2 gas in steel cylinder
Pressure surge tank, 2gal
Orifice foam flashing apparatus
Lab glassware
Beer 23 – Beer Bitterness
Beer 23A – Bitterness Units
Various reagents
Mechanical shaker, wrist action/platform type
Precision spectrophotometer
Centrifuge+tubes
Beer 23B – Iso alpha acids (IAAs) by solvent extraction
Various reagents
Various labware
Same as 23A hardware
Beer 23C – ISAA by solid phase extraction and HPLC
Various reagents
HPLC w/UV detector and 20uL injection loop
C8 SPE octyl column, 500mg, 3mL
Integrator
Chromatographic column, shimadzu, shim-pack CLC-ODS, 25cmx4.6mm
Beer 23D – Bitterness by automated flow analysis
Various reagents
Automated flow analyzer, configured for bitterness
UV detector with flow cell, 275nm detect/6nm bandpass
Lab glassware
Beer 23E – ISAA by HPLC
Various reagents
HPLC w/column heater and UV detector
Analytical column, C18, 250x4mm (Nucleosil 5, or eq.) or 125x4mm (nucleodur 100-5
C18)
Beer 23F – IAA
Various reagents
Mechanicak shaker
Centrifuge+tubes
Various lab glassware
Beer 25 – Diacetyl
Beer 25B – Broad spectrum method for VDK
Alpha-naphthol son
KOH-creatine soln
Diacetyl, stock soln
Diacetyl, working soln
Colorimeter/specrophotometer (Appendix IA)
Distillation equipment, all glass (boiling flask, 2-neck, 500mL; distilling tube, mounted
vertically; condenser, water cooled; curved tapered tube adapter; receiver, 50ml beaker
Heating mantle for boiling flask
Other glassware
Beer 25C – Micro dimethyl glyoxime method
Various reagents
Colorimeter/spectrophotometer
Generator trap assy
Tygon tubing
Beer 25F – Gas chromatographic method B
Reagent water, deionized, distilled, or 18 MΩ, CAS# 7732-18-5
Ethanol, anhydrous, 200 proof, CAS# 64-17-5
Copper (II) sulfate pentahydrate, CAS# 7758-99-8
2,3-Butanedione, 99%, CAS# 431-03-8
2,3-Pentanedione, 97%, CAS# 600-14-6
2,3-Hexanedione, technical grade, 90%, CAS# 3848- 24-6
Hydrochloric acid, concentrated, 37%, CAS# 7647- 01-0
Gas chromatograph, equipped with a headspace autosampler and ECD
Column, capable of giving baseline separation of 2,3-butanedione, 2,3-pentanedione,
and 2,3-hexanedione, such as a Restek Rtx-5 column (15 m, 0.32 mm ID, 1.5 µm df) (Cat.#
10266) or similar
Vials and caps, compatible with autosampler
Orbital mixer
Volumetric flasks and stoppers, 50, 100, 250, and 500 mL, Class A
Volumetric pipette, 25 mL
Pipettes, capable of delivering 50–200, 100–1,000, and 1,000–10,000 µL.
Beer 26 – Formazin turbidity standards
Various reagents
Nephelometer, (optional), capable of 580nm measurements
Various lab glassware
Beer 27 – Physical Stability
I. Total haze after chilling
a. Visual method
i. Formazin turbidity standards
ii. Clark turbidimeter model T1
iii. Red plexiglass sheet
iv. Temp bath
v. Flint glass bottles
vi. Clear drinking glasses
b. Nephelometric method
i. Formazin turbidity standard
ii. Nephelometer
iii. Thermometer
II. Accelerated chill-haze test
a. Elevated temperature before chilling
i. Incubator/bath
Beer 29 – Lower boiling volatiles in beer or ale
Various reagents
Various glassware
Laboratory bottle crowner
GC with FID
Column, 15ftx1/8in stainless steel with 20% carbowax 20M on chromosorb W (HDMS) 60/80
mesh
Beer 32 – Viscosity
View Beer1A and Wort 13
Beer 36 – Sodium by atomic absorption spectrophotometry
Various reagents
Atomic absorption spectrophotometer, sodium hollow-cathode lamp
Various glassware
Beer 37 - Potassium by atomic absorption spectrophotometry
Various reagents
Atomic absorption spectrophotometer, potassium hollow-cathode lamp
Various glassware
Beer 38 – Magnesium and calcium
Beer 38A – Magnesium by atomic absorption spectrophotometry
Various reagents
Atomic absorption spectrophotometer
Magnesium hollow cathode lamp
Labware
Beer 38B – Calcium and magnesium by sequential titration
Various reagents
Labware
Microburet, 10mL
Beer 41 – Total Carbohydrates
Beer 41B
Various reagents
HPLC with refractive index detector, column heater, electronic integrator, autosampler
Chromatographic column, Bio-Rad fruit quality analysis column, 100x7.8mm hydrogen-form
cation exchange resin-based column
Aliquot mixer, Thermolyne M126125
Filter system, disk filter holders
Beer 38 – Headspace GC FID analysis of Beer volatiles
Various reagents
Static headspace autosampler, Teledyne Tekmar, Model 7000
GC w/ FID
Column, stabilWax 60mx0.32mmID x1um film thickness
Vortex mixer
Autosampler vials, 20mL borosilicate glass
Crimp top septa, aluminum ring with teflon septa
Syringes, air-tight, 100, 250, 500uL
