Explode-A-Grape Lab
Introduction: Diffusion is the movement of particles from an area of high
concentration to an area of low concentration. Osmosis is the diffusion of water,
often across a semipermeable membrane, such as the cell membrane, or grape
skin. Solutions that are hypertonic cause water to leave the cell (or grapes), while
hypotonic solutions cause water to flood inside of the cell (or grapes). Osmotic
pressure can sometimes be so great that membranes (or grapes) burst.
Objective: Students will witness and understand osmosis and diffusion.
Materials (per group):
o Two grapes o Two plastic cups
o Distilled water o 3 to 4 tsp. Sodium
o Graduated cylinder chloride
o Beaker o Masking tape
o Measuring tape o Stir sticks
Directions:
1. FRIDAY: Mix the sodium chloride with the 100 mL distilled water in the
glass beaker. Mix in as much salt as will dissolve – the more the better!
Use the stir stick to make sure you water is fully saturated with sodium
chloride.
2. Label one of the cups “fresh” and the other one “saline.”
3. Measure and record the circumference of each grape and record it in the
table below.
4. Measure and record the mass of each grape and record it in the table
below.
5. Place one grape in each cup.
6. Fill each cup with either saline solution or 100 mL distilled water,
depending on labels.
7. Find a safe place in the back of the classroom to store your cups for the
weekend.
8. Make sure you put your names on your cups so you know which one is
yours on Monday.
9. MONDAY: Dump your water out in the sinks and gently pat dry your grapes
(try not to squish them).
10.Measure and record the new circumference of each grape and record it in
the table below.
11.Measure and record the new mass of each grape and record it in the table
below.
Data Table
Initial Initial Mass (g) Final Final Mass (g)
Circumference Circumference
(mm) (mm)
Grape – Fresh
Water
Grape – Saline
Water
Conclusion
1. Which grape ruptured? Did you expect this? Why or why not?
2. Which grape shriveled? Did you expect this? Why or why not?
3. Which cup contained the hypertonic solution? How can you tell?
4. Which cup contained the hypotonic solution? How can you tell?
5. In your own words, explain why your grape exploded (on a molecular level,
what happened?).
6. What do you think might happen if the cells in your body were exposed to
ocean water?