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Task 1

The lab report identifies unknown bacteria D as Escherichia coli. Gram staining of the bacteria showed purple rods, indicating a Gram-negative result. Citrate and SIM tests produced green and brown colors respectively, confirming the presence of E. coli. MSA testing also showed fermentation, a positive result for E. coli. While many E. coli strains are harmless, some can cause food poisoning and other infections like urinary tract infections and pneumonia. The lab tests and observations identified the unknown bacteria as the common intestinal bacterium Escherichia coli.

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71 views4 pages

Task 1

The lab report identifies unknown bacteria D as Escherichia coli. Gram staining of the bacteria showed purple rods, indicating a Gram-negative result. Citrate and SIM tests produced green and brown colors respectively, confirming the presence of E. coli. MSA testing also showed fermentation, a positive result for E. coli. While many E. coli strains are harmless, some can cause food poisoning and other infections like urinary tract infections and pneumonia. The lab tests and observations identified the unknown bacteria as the common intestinal bacterium Escherichia coli.

Uploaded by

Dennis Mwangi
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as DOCX, PDF, TXT or read online on Scribd
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Surname 1

Name:

Tutor:

Course:

Date:

Lab Report: Bacteria

Unknown Letter: D

Bacteria Identified as: Escherichia coli

Introduction

There are several methods that can be used in identification of unknown bacterial in a

laboratory test. The methods are discrete and hence reliance on one can give a biased output.

This laboratory experiment aimed at establishing the identity of unknown bacteria D. The

methods used include Gram stain test, MSA test, citrate test and SIM test.

Materials and Methods

The experiment needed materials including sterile swab, microscope, Gram stain, heat

source, culture and microscope slides. For the Gram stain test, a smear was prepared which was

applied on to the primary stain which appeared crystal violet. This process was then followed by

the application of the mordant, Iodine which distinguished the orange and purple colors. Excess

iodine was removed by rinsing and decolorization done using +20% acetone and 80% methanol

for 5 seconds. Using safranin, a counterstain was performed for 2 minutes and the outcome

rinsed, dried and observations done which were purple and pink colors. The other tests were

carried out distinctly and observations made for the similar sample.

Discussion
Surname 2

The procedure of the laboratory report resulted to regular outputs presented according to

the tests that were carried out. For Gram stain test, the following diagram was observed on the

microscope

Gram Stain Result

According to the Gram stain output, the bacteria was identified to be Escherichia coli

which is known to turn purple for Gram stain positive results (Nesher, L., et al 193).

The other tests’ results are as shown below

Citrate Result SIM Result


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In citrate results, the output was seen as green which is an indication of presence of

Escherichia coli bacteria in the sample (Nesher, L., et al 194). SIM test resulted to brown color

which confirms presence of Escherichia coli bacteria (Nesher, L., et al 194).

MSA Result

MSA test was carried out and the results indicated positive for Escherichia coli since it

fermented the sample.

Conclusion

Escherichia coli is a type of bacteria that usually resides in the intestines. It is found in

some animals' stomach, too (Monk et al. 904). Many E. coli organisms are harmless, and also

help preserve healthy digestive tracts. Consumption of contaminated food, or drink fouled water,

some strains can cause diarrhea due to the E. coli bacteria (Monk et al. 905). E. coli bacteria are

associates with food poisoning. Pneumonia and urinary tract infections can also be caused by the

bacteria. In addition, E. coli is responsible for other infection in the body. About 75 per cent to

95 per cent of urinary tract infections are from E. coli.


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Works Cited

Monk, Jonathan M., et al. "i ML1515, a knowledgebase that computes Escherichia coli

traits." Nature biotechnology 35.10 (2017): 904-908.

Nesher, L., et al. "Epidemiology of meningitis with a negative CSF Gram stain: under-utilization

of available diagnostic tests." Epidemiology & Infection 144.1 (2016): 189-197.

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