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DNS Method for Sucrose Assay Analysis

The document discusses using the dinitrosalicylic (DNS) colorimetric method to assay sucrose. DNS reacts with reducing sugars like glucose and fructose to produce a red-brown color. Test tubes containing hydrolyzed glucose solutions were analyzed with a spectrophotometer and found to have various mol/L concentrations. However, the standard curve plotted from the data did not show a linear trend, indicating issues with the experiment such as defective reagents or equipment.

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Thonieroce Apryle Jey Morelos
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544 views1 page

DNS Method for Sucrose Assay Analysis

The document discusses using the dinitrosalicylic (DNS) colorimetric method to assay sucrose. DNS reacts with reducing sugars like glucose and fructose to produce a red-brown color. Test tubes containing hydrolyzed glucose solutions were analyzed with a spectrophotometer and found to have various mol/L concentrations. However, the standard curve plotted from the data did not show a linear trend, indicating issues with the experiment such as defective reagents or equipment.

Uploaded by

Thonieroce Apryle Jey Morelos
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
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INTERPRETATION AND DISCUSSION

Sucrose Assay Using Dinitrosalicylic (DNS) Colorimetric Method

Reducing sugars reacts or binds only to Dinitrosalicylic (DNS) (example: invert sugar - a mixture of two
simple sugar, glucose and fructose). In the experiment, as the dinitrosalicylic acid, was added in each of the
test tubes, through immersing it in a boiling water bath, due to the presence of heat, it will give the
characteristic of red-brown color. This is because the conversion of the 3,5 dinitrosalicylic acid to 3-amino-5-
nitrosalicylic acid, and the glucose, a product from invertase activity converted to gluconic acid which
contributed to the red-brown color of the mixture. The concentration (mol/L) of each hydrolyzed glucose on
test tubes was identified using the spectrophotometer.

Test Tube 1: 48.0706 mol/L

Test Tube 2: 398.9743 mol/L

Test Tube 3: 86.4318 mol/L

Test Tube 4: 398.9743 mol/L

Test Tube 5: 398.9743 mol/L

Test Tube 6: 71. 6499 mol/L

Test Tube (Blank): 0.4073 mol/L

[GRAPH]

Upon constructing the invert sugar standard curve by plotting, a linear trend was not shown in the graph or
the “best fit” was not identified. This is due to some possible factors like the dinitrosalicylic acid might not be
reactive, the inverted sugar is not properly stored at its appropriate temperature or the spectrophotometer was
defective or not sensitive enough, fails in reading and may actually have errors seeing that some of the
performed test on the other experiment shows no concentration, even after re-running the test.

CONCLUSION

DNS colorimetric method is used in the experiment for the determination of reducing sugar.
The DNS method was specifically used in the experiment to determine the reducing sugar
concentration of the inverted sugar using the spectrophotometry. The gathered data does not met the
“best fit” or the linear trend proving the experiment to be specific, validated, accurate or precise.
Therefore, this only means that DNS does not satisfy the experiment for the reducing sugar. This is
due to some possible factor that affects the invertase such as the temperature and pH, DNS used, or
defects in spectrophotometry. But the adding of DNS reagent develops a right color characteristic
(red-brown color) due to the presence of heat.

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