Thermo Scientific

Titan3 and Target2

Chromatography Syringe Filters

Performance filtration
solutions
for sample preparation
 Titan3 and Target2
syringe filters
Sample preparation is a key stage
in successful chromatography.
Thermo Scientific Titan3 and
Target2 syringe filters ensure
reliable elimination of both
particles and micro organisms in
the sample preparation process,
providing consistent and reliable
experimental results for a range of
samples and applications. Titan3
and Target2 syringe filters can also
protect chromatography columns
by preventing the cumulation of
fine particles in the column, which
may form premature blockages.

2
Both Titan3 and Target2 provide
high-quality filtration solutions

• Low extractable membranes and housing

• HPLC performance tested
• Plain polypropylene housing
• 30 mm products pressure rated to 100 psi
• Enhanced Luer Lock inlet which provides secure
attachment to syringes
• Range of filter sizes 4 mm – 30 mm
• Low hold-up volume allows for the treatment of
small sample volumes with minimal sample loss

The premium Titan3 range

provides even higher levels of
confidence

• Integral ring provides greater strength to the

housing, preventing leakage and bursting
• 30 mm products pressure rated to 120 psi
• Most 30 mm devices are provided with a 1 mm
boro silicate glass pre-filter (delivering increased
filtrate volumes)
• Color coding for easy identification and selection of
the correct membrane and pore size

3
Titan3 and Target2

2
Syringe filter membrane
selection Compatibility of membrane with
The following is a general guide when selecting the appropriate
solvent
membrane for your application. The following should be
considered: Water /Soluble Organic

1
• CA • PTFE
• Nylon • PTFE-L
Membrane compatibility with • PES • PVDF
sample type and technique • PTFE-L • RC

3
• Aggressive and nonpolar solvents PTFE
• Organic/Aqueous Solvents PTFE-L
Filter size is dependent on sample
• Biological sample preparation RC
volume
• Capillary electrophoresis PES
• High solid samples GMF/pre-filter • <2 mL 4 mm
• HPLC PVDF/Nylon/RC/ • <10 mL 17 mm
PTFE-L • <100 mL 30 mm
• Ion-chromatography PES/PVDF

4
• Protein analysis PVDF
• Trace metals PES Porosity depends on the amount
• UV-Vis spectophotometry PVDF of particles to be removed from
the sample

• >5 µm GF pre-filter
For more detailed information on individual • <2 µm 0.45 µm
membrane specifications, please see pages 5 - 9.
• <0.5 µm 0.2 µm

Titan3 Syringe filter unit specification Target2 Syringe filter unit specification

4 mm Titan3 17 mm Titan3 30 mm Titan3 4 mm Target2 17 mm Target2 30 mm Target2

Polypropylene with Polypropylene with Polypropylene with

Housing integral colour-coded integral colour-coded integral colour-coded Housing Polypropylene Polypropylene Polypropylene
sealing ring sealing ring sealing ring
Filtration area 0.125 cm2 1.6 cm2 4.9 cm2 Filtration area 0.125 cm2 1.6 cm2 4.9 cm2
Maximum pressure 5.5 BAR 8 BAR 10 BAR Maximum 5.5 BAR 8 BAR 10 BAR
pressure
Residual volume <15 µL with air purge <35 µL with air purge <157 µL with air purge Residual volume <15 µL with air purge <35 µL with air purge <157 µL with air purge
Dimensions 8 mm × 20.7 mm 20.5 mm × 24.5 mm 34 mm × 24.5 mm Dimensions 8 mm × 20.7 mm 20.5 mm × 24.5 mm 34 mm × 24.5 mm
Flow direction Inlet-outlet only Inlet-outlet only Inlet-outlet only Flow direction Inlet-outlet only Inlet-outlet only Inlet-outlet only
Inlet Female luer lock tab Female luer screw Female luer screw Inlet Female luer lock tab Female luer screw Female luer screw
Outlet Male luer slip Male luer slip Male luer slip Outlet Male luer slip Male luer slip Male luer slip
Pre-filter N Selected membranes Selected membranes Pre-filter N N Selected membranes

4
 Titan3 and Target2

Syringe filter membrane

specification

PVDF syringe filters

Superior membrane quality for HPLC.
• Low non-specific binding with exceptional chemical resistance
• Hydrophilic membrane provides excellent flow rates and low
binding coefficients
• Certified for chromatographic performance
• Compatible with a wide range of aqueous and organic-based
sample environments
• Recommended for HPLC and UHPLC

PVDF syringe filters have a well­ defined pore structure, giving

effective retention of particles without excessive pore blockage.
Compatible with aqueous and most organic solvents. They are
excellent general filters for HPLC and organic solvent sample
clean up.

Specifications
Membrane: HPLC certified PVDF
Max. operating 100°C
temperature:
Housing: medical grade, virgin polypropylene
Pre-filter: binder free glass microfiber 1 µm
(0.20 µm only)
Porosities: 0.20 µm, 0.45 µm
Autoclave: Sterilize by dry heat at 121°C for 15 minutes

Applications
• HPLC and organic solvent sample preparation and clean up
• Protein based samples with high non-specific binding
• Environmental water samples

Chemical Incompatibilities
• DMF, DMSO, MEK, acetone and most caustic solutions >6N

5
Titan3 and Target2

Regenerated cellulose syringe Cellulose acetate syringe filters

filters
Superior chemical resistance, optimized for biological sample For filtering of aqueous solutions or biological samples.
recoveries. • Hydrophilic membrane provides excellent flow rates and
• Hydrophilic membrane provides excellent flow rates and extremely low protein-binding coefficients
extremely low binding coefficients • Superior choice for biological assays, gel capsule dissolution
• Superior choice for biological assays, gel capsule testing, protein sample matrixes
dissolution testing, protein sample matrixes • Recommended for aqueous HPLC
• Compatible with a wide range of aqueous and
organic-based sample environments
A physically strong membrane which can be used with heated
• Recommended for reverse phase and normal phase HPLC liquids.

Regenerated cellulose syringe filters are ideally suited for almost Specifications
any laboratory procedure, from HPLC sample preparation to
dissolution sample testing. Regenerated cellulose possesses Membrane: HPLC certified cellulose acetate
superior chemical resistance in either aqueous or organic-based Max. operating 110°C
sample environments. Its extremely low biological-based binding temperature:
coefficient is ideally suited for maximum sample recoveries
Protein binding: <24 µg/cm2
of biological-based assays. Regenerated cellulose contains
no binders, surfactants or wetting agents to assure minimal Porosities: 0.20 µm, 0.45 µm
extractables in analytical procedures. Autoclave: Sterilize by dry heat at 121°C for 15 minutes

Specifications Applications
Membrane: HPLC certified regenerated cellulose • Protein-based samples with high non-specific binding
Protein binding: <5 µg/cm2 • Sample analysis which require maximum recoveries
Porosities: 0.20 µm, 0.45 µm
Autoclave: sterilize by dry heat at 121°C for 15 minutes Chemical Incompatibilities
Pre-filter (if fitted) GMF 1 µm • Acids, NaOH, dichloromethane, chloroform, ketones, DMSO,
THF
Applications
• HPLC and organic solvent sample preparation and clean up
• Dissolution sample analysis, especially high-binding tablets or
capsules
• Protein-based samples with high non-specific binding
• Sample analysis which require maximum recoveries
• Analysis requiring low non-specific binding over a wide pH
range

Chemical Incompatibilities
• Sulfuric acid, hydrochloric acid, phosphoric acid or nitric acid
>25%, DMF, phenol

6
 Titan3 and Target2

Polypropylene syringe filters Glass microfiber (GMF) syringe

filters
Chemically resistant membrane with low protein binding. For large particulate removal.
• Hydrophilic membrane for aqueous or organic sample • Increased sample throughput
matrixes • Low extraction neutral borosilicate glass
• Use with protein or peptide-based assays • For use with viscous or particle-laden samples

The hydrophilic polypropylene membrane is easily wetted GMF filters are available in a range of porosities.
with water and does not require pre-treatment with alcohols.
Compatible with biological samples. Do not use with strong
organic solvents, especially aromatic and chlorinated solvents. Specifications
Membrane: binder free glass microfiber
Specifications Max. operating 110°C
Membrane: hydrophilic polypropylene temperature:
Max. operating 110°C Housing: medical grade, virgin polypropylene
temperature: Porosities: 0.7 µm, 1.2 µm, 3.1 µm
Housing: medical grade, virgin polypropylene Autoclave: sterilize by dry heat at 121°C for 15 minutes
Porosities: 0.20 µm, 0.45 µm
Applications
Applications • Clarification
• Protein or peptide-based assays • Pre-filtering of suspensions
• General HPLC analysis
Chemical Incompatibilities
Chemical Incompatibilities • Limited resistance with ammonia, NaOH and KOH solutions
• Hexane, toluene, benzene, limited resistance to
dichloromethane and chloroform

7
Titan3 and Target2

PTFE syringe filters Hydrophilic PTFE syringe filters

Excellent chemical resistance for use with organic matrices. Excellent chemical resistance for use with organic and aqueous
• Naturally hydrophobic membrane provides excellent flow rates matrices.
and high loading capacities • Modified hydrophilic membrane provides excellent flow rates
• Exceptional temperature stability and high loading capacities

• Organic solvent recommended • Exceptional temperature stability up to 130°C

• Aqueous/organic solvent recommended

PTFE syringe filters are applicable for filtration of gaseous

or organic solvent-based samples. The natural hydrophobic Hydrophilic PTFE (PTFE-L) syringe filters are applicable for filtration
membrane exhibits broad chemical resistance and unsurpassed of gaseous or organic solvent-based samples. The modified
temperature stability to address aggressive sample matrixes membrane exhibits broad chemical resistance and unsurpassed
and extreme temperature situations. Due to the membrane’s temperature stability to address aggressive sample matrixes and
hydrophobic nature, PTFE filters can be utilized as a moisture extreme temperature situations.
barrier in venting applications. Low protein binding membrane
PTFE membranes require pre-treatment with alcohol before being The hydrophilic PTFE membranes are suitable for filtration of
suitable for aqueous or high aqueous/organic samples. Do not use aqueous or highly aqueous/organic samples. There is no need
directly with aqueous solutions. for pre-treatment of the membrane prior to use with aqueous
samples.
Specifications The membrane has extremely low levels of ionic extractibles and
Membrane: HPLC certified PTFE, w/polypropylene may be used for Ion chromatography analysis of inorganic and
support organic ions.

Max. operating 110°C

temperature: Specifications
Housing: medical grade, virgin polypropylene Membrane: HPLC certified Hydrophilic PTFE,
Porosities: 0.20 µm, 0.45 µm, 1 µm w/polypropylene support

Autoclave: sterilize by dry heat at 121°C for 15 minutes Max. operating 130°C
temperature:
Housing: medical grade, virgin polypropylene
Applications
Porosities: 0.22 μm, 0.45 μm
• HPLC and organic solvent sample preparation and clean up
Autoclave: sterilize at heat setting 121°C for
• Dissolution sample analysis 15 minutes
• General sample preparation prior to analytical analysis
• Elevated temperature samples, caustic or acidic solutions Applications
• HPLC aqueous and organic solvent sample preparation and
Chemical Incompatibilities clean up
• Perchloric acid • Dissolution sample analysis
• Methylene chloride (limited exposure) • General sample preparation prior to analytical analysis
• Dioxane, DMF, formic acid >50% • Elevated temperature samples, caustic or acidic solutions
• Aqueous-based sample matrix (unless filter is pre-wetted with
an alcohol) Chemical Incompatibilities
• Perchloric acid
• Methylene chloride (limited exposure)
• Dioxane, DMF, formic acid >50%

8
 Titan3 and Target2

Nylon syringe filters PES syringe filters - Ion

chromatography (IC) certified
Membrane of choice for analytical applications. Precise results in sensitive analysis of ionic analytes.
• Naturally hydrophilic with broad chemical resistance • Certified for low-level IC interference by ICP analysis
• Excellent flow rates and high-throughput loading • Hydrophilic membrane provides excellent flow rates and low
• HPLC recommended binding coefficients
• Low affinity for binding drugs, ideal for dissolution testing
procedures
Hydrophillic nylon is extremely well suited for aqueous or organic
sample preparation and HPLC, GC or dissolution sample analysis.
Due to its excellent flow characteristics and mechanical stability, This hydrophilic polymer has excellent cleanliness and is
nylon offers the best combination of physical parameters to meet compatible with a wide range of solvents. It is the membrane of
the most stringent analytical needs. choice for ion chromatography applications.

Specifications Specifications
Membrane: HPLC certified nylon Membrane: ICP Certified PES (PolyEtherSulfone)
Max. operating 100°C Max. operating 100°C
temperature: temperature:
Housing: medical grade, virgin polypropylene Housing: medical grade, virgin polypropylene
Porosities: 0.20 µm, 0.45 µm, 1.5 µm, 5 µm Pre-filter: binder-free glass microfiber pre-filter
Autoclave: sterilize by dry heat at 121°C for 15 minutes (0.20 µm only)
Porosities: 0.20 µm, 0.45 µm

Applications Autoclave: Sterilize by dry heat at 121°C for 15 minutes

• HPLC and organic solvent sample preparation and clean up

• Dissolution sample analysis Applications

• General sample preparation prior to analytical analysis • IC sample preparation and analysis

• Mixed sample matrix of aqueous or organic dissolved analytes • Dissolution testing

Chemical Incompatibilities Chemical Incompatibilities

• Acids >1N • Protein-based samples in aqueous solutions

Halogenated solvents • Concentrated acids, chloromethane, chloroform, hexane,

acetone, MEK, THF, DMSO
• Proteineous samples with high non-specific binding affinities

Analysis results (ppb)

Method detection
Analyte 0.2µm PES 0.45µm PES
level (ppb)
Bromide < 100 < MDL < MDL
Chloride 20 < MDL < MDL
Fluoride 20 < MDL < MDL
Nitrate as N 50 < MDL < MDL
Orthophosphate as P < 100 < MDL < MDL
Sulfate < 100 < MDL < MDL
Barium 2 < MDL < MDL
Calcium 10 39.9 58.9
Potassium 50 80 70
Magnesium 20 < MDL < MDL
Sodium 20 80.6 63

9
Titan3 and Target2

Cross reference Thermo Scientific

Titan3 Ring color
Thermo Scientific
Target2
syringe filter units Product code Product code
4 mm syringe filter 0.45 µm nylon 44504-NN Transparent Green F2504-1
4 mm syringe filter 0.2 µm polypropylene N/A F2504-10
4 mm syringe filter 0.45 µm cellulose acetate N/A F2504-15
4 mm syringe filter 0.2 µm cellulose acetate 44502-CA Light Green F2504-16
4 mm syringe filter 0.2 µm nylon 42204-NN Opaque Medium Yellow F2504-2
4 mm syringe filter 0.45 µm PTFE 44504-NP Transparent Yellow F2504-3
4 mm syringe filter 0.2 µm PTFE 42204-NP Transparent Blue F2504-4
4 mm syringe filter 0.45 µm PVDF 44504-PV Red F2504-5
4 mm syringe filter 0.2 µm PVDF 42204-PV Black F2504-6
4 mm syringe filter 0.45 µm regenerated cellulose 54504-RC Light Brown F2504-7
4 mm syringe filter 0.2 µm regenerated cellulose 52204-RC Granite F2504-8
4 mm syringe filter 0.45 µm polypropylene N/A F2504-9

17 mm syringe filter 0.45 µm nylon 44513-NN Transparent Green F2513-1

17 mm syringe filter 0.2 µm polypropylene 42213-PP Royal Blue F2513-10
17 mm syringe filter 0.45 µm PES for Ion chromatography 44513-PS Orange/Yellow F2513-14
17 mm syringe filter 0.45 µm cellulose acetate 44513-CA Transparent Orange F2513-15
17 mm syringe filter 0.2 µm cellulose acetate 42213-CA Light Green F2513-16
17 mm syringe filter 0.2 µm PES for Ion chromatography 42213-PS Dark Gray F2513-17
17 mm syringe filter 0.2 µm nylon 42213-NN Light Purple F2513-2
17 mm syringe filter 0.45 µm PTFE 44513-NP Transparent Yellow F2513-3
17 mm syringe filter 0.2 µm PTFE 42213-NP Transparent Blue F2513-4
17 mm syringe filter 0.45 μm hydrophilic PTFE 44513-NPL Purple
17 mm syringe filter 0.2 μm hydrophilic PTFE 42213-NPL Green
17 mm syringe filter 0.45 µm PVDF 44513-PV Red F2513-5
17 mm syringe filter 0.2 µm PVDF 42213-PV Black F2513-6
17 mm syringe filter 0.45 µm regenerated cellulose 54513-RC Light Brown F2513-7
17 mm syringe filter 0.2 µm regenerated cellulose 52213-RC Granite F2513-8
17 mm syringe filter 0.45 µm polypropylene 44513-PP White F2513-9

30 mm syringe filter 0.45 µm nylon with pre-filter 44525-NN Transparent Green F2500-1
30 mm syringe filter 0.2 µm polypropylene 42225-PP Royal Blue F2500-10
30 mm syringe filter 5.0 µm PTFE N/A F2500-11
30 mm syringe filter 1.25 µm nylon 41225-NN Opaque Medium Yellow F2500-12
30 mm syringe filter 1.0 µm PTFE 41025-NP Dark Blue F2500-13
30 mm syringe filter 0.45 µm PES for Ion chromatography 44525-PS Orange/Yellow F2500-14
30 mm syringe filter 0.45 µm cellulose acetate 44525-CA Transparent Orange F2500-15
30 mm syringe filter 0.2 µm cellulose acetate 42225-CA Light Green F2500-16
30 mm syringe filter 0.2 µm PES for Ion chromatography with pre-filter 42225-PS Dark Gray F2500-17
30 mm syringe filter 0.7 µm glass micro fiber 40725-GM Amber F2500-18
30 mm syringe filter 1.2 µm glass micro fiber 41225-GM Orange F2500-19
30 mm syringe filter 0.25 µm nylon 45025-NN Pink F2500-2
30 mm syringe filter 3.1 µm glass micro fiber 42725-GM Dark Purple F2500-20
30 mm syringe filter 0.45 µm PTFE with pre-filter 44525-NP Transparent Yellow F2500-3
30 mm syringe filter 0.2 µm PTFE with pre-filter 42225-NP Transparent Blue F2500-4
30 mm syringe filter 0.45 µm PVDF 44525-PV Red F2500-5
30 mm syringe filter 0.45 µm nylon 44526-NN White F2500-50
30 mm syringe filter 0.2 µm PVDF 42225-PV Black F2500-6
30 mm syringe filter 0.45 µm regenerated cellulose 54525-RC Light Brown F2500-7
30 mm syringe filter 0.2 µm regenerated cellulose 52225-RC Granite F2500-8
30 mm syringe filter 0.45 µm polypropylene 44525-PP White F2500-9
Alternative parts are based on a direct
30 mm syringe filter 0.45 µm nylon 44525-NN Transparent Green F2502-1
technical comparison.
30 mm syringe filter 0.2 µm nylon 42225-NN Light Purple F2502-2
Part number alternatives are based upon 30 mm syringe filter 0.20 µm polypropylene 42225-PP Royal Blue F2502-10
closest pack quantity. 30 mm syringe filter 0.45 µm PTFE 44525-NP Transparent Yellow F2502-3
Inclusion of parts is no guarantee of 30 mm syringe filter 0.45 μm hydrophilic PTFE 44525-NPL Purple
identical performance. 30 mm syringe filter 0.2 μm hydrophilic PTFE 42225-NPL Green
30 mm syringe filter 0.45 µm polypropylene 44525-PP White F2502-9
10
GE Healthcare/Whatman GE Healthcare/Whatman Millipore PALL Sartorius
GD/X Puradisc Spartan Millex Acrodisc Minisart
6789-0404 SLHNR04NL 4484
6788-0402

6789-0402 SLGNR04NL
6783-0404 SLFHR04NL 4472 17820-K
6783-0402 SLFGR04NL 17573-K2
6779-0404 SLHVR04NL
6779-0402 SLGVR04NL 4415
17822-K
17821-K
6788-0404

6870-1304 6789-1304 SLHN013NL 4426 17762-K

6788-1302 4567
6782-1304
6880-1304
6880-1302
6876-1302 6782-1302
6870-1302 6789-1302 SLGN013NL 4427 1776B-K
6874-1304 6783-1304 SLFH013NL 4422 17574-K
6874-1302 6783-1302 SLFG013NL 4423
SLCRT13NL
SLLGH13NK MS-3301
6872-1304 6779-1304 SLHV013NL 4457
6872-1302 6779-1302 SLGV013NL 4455
17762-K
17761-N
6784-1304 6788-1304 4563

6870-2504 6750-2502 6710-2504 SLHN025NS 4438 1784C-K

6785-2502 4564

6878-2504 6780-2504 6716-2504 SLHP033NS 4584

6880-2504 17598-K
6880-2502 17597-K
6878-2502 6780-2502 SLGP033NS
6890-2507
6886-2512
6870-2502 6750-2502 SLGN025NS 4436 1784B-K
6888-2527
6874-2504 6784-2504 6714-2504 SLFH025NS 4219 17576-K
6874-2502 6784-2502 SLFG025NS 4225 17575-S
6872-2504 6712-2504 SLHV033NS 4408

6872-2502 SLGV025NB 4406

17765-K
17764-S
6718-2504 4560
6870-2504 4549
6871-2502 4436 1784B-K
6878-2502 4307
6874-2504 4303
SLCR025NB
SLLGDZ5NK MS-3201
6878-2504 4559
11
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BR20825_E 07/13S