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Sant Tukaram National Model School, Latur: Shiv Chhatrapati Shikshan Santha's

This document provides instructions for preparing a stained temporary mount of human cheek cells under a microscope. The objective is to observe and record the characteristics of cheek cells. The procedure involves scraping cheek cells and placing them on a slide, staining with methylene blue and glycerin, then observing under low and high magnification. Observations show many flat, oval or irregular cells surrounded by a cell membrane enclosing cytoplasm and a dense oval nucleus, without a cell wall as in animal cells. Precautions are taken to gently scrape cheeks and properly prepare and observe the slide.

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Samarth Mandade
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48 views9 pages

Sant Tukaram National Model School, Latur: Shiv Chhatrapati Shikshan Santha's

This document provides instructions for preparing a stained temporary mount of human cheek cells under a microscope. The objective is to observe and record the characteristics of cheek cells. The procedure involves scraping cheek cells and placing them on a slide, staining with methylene blue and glycerin, then observing under low and high magnification. Observations show many flat, oval or irregular cells surrounded by a cell membrane enclosing cytoplasm and a dense oval nucleus, without a cell wall as in animal cells. Precautions are taken to gently scrape cheeks and properly prepare and observe the slide.

Uploaded by

Samarth Mandade
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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Shiv Chhatrapati Shikshan Santha’s

Sant Tukaram National Model School, Latur

1
Experiment
• Objective

To prepare a stained
temporary mount of human
cheek cells and to record
observations and draw
labelled diagrams
Apparatus and Materials Required
• Toothpick, slide, coverslip, filter
paper, needles, brush, watch glass,
methylene blue, dropper, glycerin,
water and a compound
microscope
Theory
• Animal cells are usually irregular in shape. They do
not have a cell wall. They are surrounded by a cell
membrane and contain cytoplasm and nucleus.
Procedure
• 1. With the help of the flat end of a washed
toothpick gently scrape the inside of your cheek.
• 2. Place the scrapings in the centre of a clean glass
slide.
• 3. Add a drop of water and a drop of methylene
blue.
• 4. After one minute remove the extra water mixed
with methylene blue by slightly tilting the slide.
• 5. Put a drop of glycerine over the stained scrapings
and cover it gently with a coverslip.
• Remove the excessive glycerine using filter paper.
• 7. Observe the scrapings under the low and high
magnifications of a microscope.
Observation
• Many flat, oval or irregular cells are seen. The
cell membrane encloses cytoplasm and an
oval, dense nucleus.
• The cell wall is absent as in all animal cells.
Precautions
• The cheeks should be scraped gently avoiding
any injury.
• 2. Over-staining and under-staining of the cells
should be avoided.
• 3. Coverslip should be placed carefully
avoiding the entry of air bubbles.
• 4. A dry and clean glass slide and coverslip
should be used.
• 5. The cheek cells should be spread properly
to avoid their folding.

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